Copy Number and Expression Data of the Various Lines Used in This Study
| Construct . | Line . | Transgene Copy No. . | % Human γ+β/Mouse α . | Expression . | γ/γ+β mRNA % in Adults . |
|---|---|---|---|---|---|
| . | . | . | Globin mRNA (in adults) . | per Copy % . | . |
| HS2 Aγβ | M2 | 6 | 39.2 ± 8.7 | 13.1 | 17.2 ± 5.3 |
| (31.5-57.5) | (5.0-19.4) | ||||
| T1 | 32 | 34.0 ± 10.8 | 2.1 | 9.5 ± 2.4 | |
| (24.7-56.8) | (6.9-14.0) | ||||
| HS2 GγAγ−117 δβ | J14 | 3 | 37.5 ± 7.5 | 25.0 | 4.7 ± 2.2 |
| (28.1-49.2) | (2.0-13.1) | ||||
| J17 | 7 | 31.3 ± 8.8 | 8.9 | 6.1 ± 2.0 | |
| (25.2-43.4) | (2.5-7.9) | ||||
| J21 | 24 | 26.6 ± 5.7 | 2.2 | 5.0 ± 2.5 | |
| (20.7-35.5) | (2.1-10.4) | ||||
| HS2 GγAγ δβ | R3 | 2 | 10.4 ± 3.1 | 10.4 | 0.6 ± 0.3 |
| (6.6-15.0) | (0.3-0.9) | ||||
| R2 | 6 | 40.5 ± 16.0 | 13.5 | 1.5 ± 0.4 | |
| (13.5-67.4) | (1.0-1.9) | ||||
| HS2 Aγβ neo | N2 | 6 | 15.4 ± 5.4 | 5.1 | 2.6 ± 1.0 |
| (10.1-22.9) | (1.1-3.4) | ||||
| N3 | 8 | 10.7 ± 3.3 | 2.7 | 2.6 ± 1.2 | |
| (8.2-17.1) | (1.1-3.4) | ||||
| N4 | 16 | 9.2 ± 2.1 | 1.1 | 3.4 ± 0.8 | |
| (7.2-14.0) | (1.4-4.2) | ||||
| HS2 Aγ en β | P5 | 1 | 11.7 ± 6.2 | 22.4 | 8.8 ± 3.6 |
| (5.0-24.2) | (5.5-17.5) | ||||
| P2 | 3 | 45.3 ± 7.3 | 30.2 | 8.7 ± 6.2 | |
| (32.1-57.4) | (1.5-20.5) | ||||
| P6 | 6 | 60.2 ± 11.5 | 20.1 | 10.4 ± 2.6 | |
| (32.4-78.6) | (7.3-14.8) | ||||
| P7 | 12 | 104.0 ± 29.5* | 17.3 | — | |
| (62.9-152.2) | |||||
| P2W | 40 | 153.5 ± 33.9* | 7.7 | — | |
| (104.0-209.5) | |||||
| LCR εGγAγδβ | 72 | 1 | 39.6 ± 12.7 | 79.2 | 0.2 ± 0.2 |
| (26.1-61.3) | (0.1-0.7) |
| Construct . | Line . | Transgene Copy No. . | % Human γ+β/Mouse α . | Expression . | γ/γ+β mRNA % in Adults . |
|---|---|---|---|---|---|
| . | . | . | Globin mRNA (in adults) . | per Copy % . | . |
| HS2 Aγβ | M2 | 6 | 39.2 ± 8.7 | 13.1 | 17.2 ± 5.3 |
| (31.5-57.5) | (5.0-19.4) | ||||
| T1 | 32 | 34.0 ± 10.8 | 2.1 | 9.5 ± 2.4 | |
| (24.7-56.8) | (6.9-14.0) | ||||
| HS2 GγAγ−117 δβ | J14 | 3 | 37.5 ± 7.5 | 25.0 | 4.7 ± 2.2 |
| (28.1-49.2) | (2.0-13.1) | ||||
| J17 | 7 | 31.3 ± 8.8 | 8.9 | 6.1 ± 2.0 | |
| (25.2-43.4) | (2.5-7.9) | ||||
| J21 | 24 | 26.6 ± 5.7 | 2.2 | 5.0 ± 2.5 | |
| (20.7-35.5) | (2.1-10.4) | ||||
| HS2 GγAγ δβ | R3 | 2 | 10.4 ± 3.1 | 10.4 | 0.6 ± 0.3 |
| (6.6-15.0) | (0.3-0.9) | ||||
| R2 | 6 | 40.5 ± 16.0 | 13.5 | 1.5 ± 0.4 | |
| (13.5-67.4) | (1.0-1.9) | ||||
| HS2 Aγβ neo | N2 | 6 | 15.4 ± 5.4 | 5.1 | 2.6 ± 1.0 |
| (10.1-22.9) | (1.1-3.4) | ||||
| N3 | 8 | 10.7 ± 3.3 | 2.7 | 2.6 ± 1.2 | |
| (8.2-17.1) | (1.1-3.4) | ||||
| N4 | 16 | 9.2 ± 2.1 | 1.1 | 3.4 ± 0.8 | |
| (7.2-14.0) | (1.4-4.2) | ||||
| HS2 Aγ en β | P5 | 1 | 11.7 ± 6.2 | 22.4 | 8.8 ± 3.6 |
| (5.0-24.2) | (5.5-17.5) | ||||
| P2 | 3 | 45.3 ± 7.3 | 30.2 | 8.7 ± 6.2 | |
| (32.1-57.4) | (1.5-20.5) | ||||
| P6 | 6 | 60.2 ± 11.5 | 20.1 | 10.4 ± 2.6 | |
| (32.4-78.6) | (7.3-14.8) | ||||
| P7 | 12 | 104.0 ± 29.5* | 17.3 | — | |
| (62.9-152.2) | |||||
| P2W | 40 | 153.5 ± 33.9* | 7.7 | — | |
| (104.0-209.5) | |||||
| LCR εGγAγδβ | 72 | 1 | 39.6 ± 12.7 | 79.2 | 0.2 ± 0.2 |
| (26.1-61.3) | (0.1-0.7) |
Expression values are given as mean and SD of 4 to 15 animals with the range given in parentheses below.
These values are from 9.5 to 11.5 day embryos.