Quantification of Plasma Glycocalicin, Platelet GPIbα, and GPIbβ
| Subject . | Plasma Glycocalicin (mg/L) . | Platelet GPIbα . | Platelet GPIbβ (% normal) . |
|---|---|---|---|
| . | . | (μg/mg protein) . | . |
| Normal | 1.40 ± 0.253-150 | 9.4 ± 3.13-150 | 100 |
| Father | 1.56 (111%) | 9.5 (101%) | 86 |
| Mother | 0.84 (60%) | 5.4 (57%) | 62 |
| Patient | 0.44 (31%) | 1.9 (21%) | 28 |
| Subject . | Plasma Glycocalicin (mg/L) . | Platelet GPIbα . | Platelet GPIbβ (% normal) . |
|---|---|---|---|
| . | . | (μg/mg protein) . | . |
| Normal | 1.40 ± 0.253-150 | 9.4 ± 3.13-150 | 100 |
| Father | 1.56 (111%) | 9.5 (101%) | 86 |
| Mother | 0.84 (60%) | 5.4 (57%) | 62 |
| Patient | 0.44 (31%) | 1.9 (21%) | 28 |
The plasma glycocalicin concentration and the GPIbα concentration in platelet lysates solubilized in Triton X-100 were quantified using an ELISA. Parentheses contain the percentages of the normal values. The platelet GPIbβ content was semiquantified by densitometry of the immunoblots probed with Gi27.
The values for normal plasma glycocalicin levels and platelet GPIbα were previously reported.22