Abrogation of In Vitro RI-AML Cell Colony Formation by Neutralizing Anti–CSF-1 and Anti–CSF-1R
| Tumor Designation . | Additions to Culture . | |||||
|---|---|---|---|---|---|---|
| . | None . | Normal Goat Serum (25 to 50 μL/dish) . | Goat Anti–Mouse CSF-1 Antiserum (50 μL/dish) . | Goat Anti–Mouse CSF-1 Antiserum + . | Mouse CSF-1 . | Goat Anti–Mouse CSF-1R Antiserum (25 μL) . |
| . | . | . | . | Human CSF-1 (60 ng/mL) . | (18 ng/mL) . | . |
| 17A-I-8 pII | 1,265 ± 310 | 1,096 ± 265 | 7 ± 4 | ND | ND | ND |
| 17A-I-31 pII | 1,386 ± 412 | 1,410 ± 307 | 0 | ND | ND | ND |
| 17E-172 pI | 535 ± 164 | 487 ± 261 | 20 ± 6 | 623 ± 202 | 764 ± 198 | 28 ± 9 |
| 17E-76 pI | 285 ± 88 | ND | 0 | ND | 783 ± 238 | 0 |
| 17E-248 pI | 294 ± 102 | ND | 22 ± 8 | 912 ± 365 | 822 ± 238 | 27 ± 17 |
| 17E-166 pI | 344 ± 108 | 322 ± 78 | 57 ± 21 | 680 ± 206 | 650 ± 202 | 50 ± 27 |
| Tumor Designation . | Additions to Culture . | |||||
|---|---|---|---|---|---|---|
| . | None . | Normal Goat Serum (25 to 50 μL/dish) . | Goat Anti–Mouse CSF-1 Antiserum (50 μL/dish) . | Goat Anti–Mouse CSF-1 Antiserum + . | Mouse CSF-1 . | Goat Anti–Mouse CSF-1R Antiserum (25 μL) . |
| . | . | . | . | Human CSF-1 (60 ng/mL) . | (18 ng/mL) . | . |
| 17A-I-8 pII | 1,265 ± 310 | 1,096 ± 265 | 7 ± 4 | ND | ND | ND |
| 17A-I-31 pII | 1,386 ± 412 | 1,410 ± 307 | 0 | ND | ND | ND |
| 17E-172 pI | 535 ± 164 | 487 ± 261 | 20 ± 6 | 623 ± 202 | 764 ± 198 | 28 ± 9 |
| 17E-76 pI | 285 ± 88 | ND | 0 | ND | 783 ± 238 | 0 |
| 17E-248 pI | 294 ± 102 | ND | 22 ± 8 | 912 ± 365 | 822 ± 238 | 27 ± 17 |
| 17E-166 pI | 344 ± 108 | 322 ± 78 | 57 ± 21 | 680 ± 206 | 650 ± 202 | 50 ± 27 |
Abbreviation: ND, not determined.
The differences between colony numbers on dishes incubated with normal goat serum and those incubated with goat anti–CSF-1 or goat anti–CSF-1R antisera are significant (P ≤ .05 by Student's t-test).