Characterization of CD4+ Th Clones
| . | Name . | Donor . | Cytokine Production* . | Surface Characteristics (mean no. of sites/cell)† . | ||||
|---|---|---|---|---|---|---|---|---|
| . | . | . | IFN-γ (U/mL) . | IL-4 (ng/mL) . | IL-10 (ng/mL) . | TCR . | CD4 . | Fas . |
| Th1 | 103 | 9923 | 32-230 | <3 | <10 | 17,770 | 93,043 | 79,750 |
| 1/36 | 1 | 213 | <3 | ND | 68,680 | 157,973 | ND | |
| 3/12 | 3 | 31-257 | <3 | <10 | 32,205 | 102,900 | 83,294 | |
| 3/21 | 3 | 46-264 | <3 | <10 | 27,037 | 144,810 | 57,489 | |
| Th2 | 3/39 | 3 | <10 | 28-75 | 33-1025 | 67,275 | 112,044 | 87,000 |
| F37 | 4 | <10 | 15-59 | ND | 63,395 | 63,395 | 62,470 | |
| 2/46 | 2 | <10 | 43-96 | ND | 18,908 | 81,486 | 15,905 | |
| . | Name . | Donor . | Cytokine Production* . | Surface Characteristics (mean no. of sites/cell)† . | ||||
|---|---|---|---|---|---|---|---|---|
| . | . | . | IFN-γ (U/mL) . | IL-4 (ng/mL) . | IL-10 (ng/mL) . | TCR . | CD4 . | Fas . |
| Th1 | 103 | 9923 | 32-230 | <3 | <10 | 17,770 | 93,043 | 79,750 |
| 1/36 | 1 | 213 | <3 | ND | 68,680 | 157,973 | ND | |
| 3/12 | 3 | 31-257 | <3 | <10 | 32,205 | 102,900 | 83,294 | |
| 3/21 | 3 | 46-264 | <3 | <10 | 27,037 | 144,810 | 57,489 | |
| Th2 | 3/39 | 3 | <10 | 28-75 | 33-1025 | 67,275 | 112,044 | 87,000 |
| F37 | 4 | <10 | 15-59 | ND | 63,395 | 63,395 | 62,470 | |
| 2/46 | 2 | <10 | 43-96 | ND | 18,908 | 81,486 | 15,905 | |
Control experiments performed on freshly isolated CD3+ T cells gave the following results (mean of three healthy donors): % TCR+ cells, 96.39 ± 1.9; mean number of TCR sites, 25,022 ± 282; % CD4+ cells, 62.3 ± 10.13; mean number of CD4 sites, 50,634 ± 2139; % CD95+ cells, 59.08 ± 6.2; mean number of CD95 sites, 12,293 ± 1,105.
Abbreviation: ND, not determined.
Value from a single determination or range from three to seven independent determinations are given.
All cells were positive for each of the surface molecules analyzed; mean number of sites/cell was calculated as described in Materials and Methods; the values reported are the mean of two to three independent determinations, variation was less than 20% of the mean.