Clonal Integration Patterns Detected in Human Hematopoietic Cells Recovered From Long-Term Engrafted bnx Mice 8.5 Months After Bone Marrow Transplantation
Mouse No. . | BMT . | No. of Integrants in Total BM . | No. of Integrants in GM Clones . | No. of Integrants in T Clones . | Common Integrant? . |
---|---|---|---|---|---|
48A1 | SNS | 0 | — | — | — |
48A2 | SNS | 0 | — | — | — |
48A3 | SNS | 0 | — | — | — |
48B1 | SNS + FL | 2 | 2 (3 + 7) | 0 | No |
48B2 | SNS + FL | 2 | 1 (8) | 1 (3) | No |
48B3 | SNS + FL | 0 | — | — | — |
48C1 | SOS | 4 | 3 (17 + 1 + 3) | 1 (1) | No |
48C2 | SOS | 0 | — | — | — |
48C3 | SOS | 6 | 5 (8 + 5 + 2 + 2 + 1) | 1 (3) | No |
Mouse No. . | BMT . | No. of Integrants in Total BM . | No. of Integrants in GM Clones . | No. of Integrants in T Clones . | Common Integrant? . |
---|---|---|---|---|---|
48A1 | SNS | 0 | — | — | — |
48A2 | SNS | 0 | — | — | — |
48A3 | SNS | 0 | — | — | — |
48B1 | SNS + FL | 2 | 2 (3 + 7) | 0 | No |
48B2 | SNS + FL | 2 | 1 (8) | 1 (3) | No |
48B3 | SNS + FL | 0 | — | — | — |
48C1 | SOS | 4 | 3 (17 + 1 + 3) | 1 (1) | No |
48C2 | SOS | 0 | — | — | — |
48C3 | SOS | 6 | 5 (8 + 5 + 2 + 2 + 1) | 1 (3) | No |
Individual human T-lymphoid (CD45+/CD3+) and myeloid (CD45+/CD33+) cells recovered from long-term engrafted mice from experiment no. 48 were deposited by ACDU into 96-well plates. A dash indicates that no human cells were recovered from the mice, so subsequent analyses were not possible. Human granulocyte-macrophage clones were grown individually in methylcellulose medium with 0.9 mg/mL G418. Human T lymphocytes were expanded individually in medium containing rHIL-2 + PHA and then shown to contain the neo gene by PCR. Clonal integration analysis by inverse PCR was performed on the total marrow sample, and each human clone recovered from each mouse. The number of proviral integrants in each category are shown as: number of integrants (no. of clones sharing the same pattern). The products of the inverse PCR reactions were analyzed to determine whether common integrants were present in the human GM and T-cell clones from each bnx recipient.