Table 6.

Clonal Integration Patterns Detected in Human Hematopoietic Cells Recovered From Long-Term Engrafted bnx Mice 8.5 Months After Bone Marrow Transplantation

Mouse No.BMTNo. of Integrants in Total BMNo. of Integrants in GM ClonesNo. of Integrants in T ClonesCommon Integrant?
48A1 SNS  —   —   —  
48A2 SNS  —   —   —  
48A3 SNS  —   —   —  
48B1 SNS + FL 2 (3 + 7) No 
48B2 SNS + FL 1 (8) 1 (3) No 
48B3 SNS + FL  —   —   —  
48C1 SOS 3 (17 + 1 + 3) 1 (1) No 
48C2 SOS  —   —   —  
48C3 SOS 5 (8 + 5 + 2 + 2 + 1) 1 (3) No 
Mouse No.BMTNo. of Integrants in Total BMNo. of Integrants in GM ClonesNo. of Integrants in T ClonesCommon Integrant?
48A1 SNS  —   —   —  
48A2 SNS  —   —   —  
48A3 SNS  —   —   —  
48B1 SNS + FL 2 (3 + 7) No 
48B2 SNS + FL 1 (8) 1 (3) No 
48B3 SNS + FL  —   —   —  
48C1 SOS 3 (17 + 1 + 3) 1 (1) No 
48C2 SOS  —   —   —  
48C3 SOS 5 (8 + 5 + 2 + 2 + 1) 1 (3) No 

Individual human T-lymphoid (CD45+/CD3+) and myeloid (CD45+/CD33+) cells recovered from long-term engrafted mice from experiment no. 48 were deposited by ACDU into 96-well plates. A dash indicates that no human cells were recovered from the mice, so subsequent analyses were not possible. Human granulocyte-macrophage clones were grown individually in methylcellulose medium with 0.9 mg/mL G418. Human T lymphocytes were expanded individually in medium containing rHIL-2 + PHA and then shown to contain the neo gene by PCR. Clonal integration analysis by inverse PCR was performed on the total marrow sample, and each human clone recovered from each mouse. The number of proviral integrants in each category are shown as: number of integrants (no. of clones sharing the same pattern). The products of the inverse PCR reactions were analyzed to determine whether common integrants were present in the human GM and T-cell clones from each bnx recipient.

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