Effect of UV Irradiation on the Viability of FDC-P1 Cells Expressing or Not Expressing mcl-1
| Transfectant Clone . | Expression of mcl-1* . | % Viable Cells . | ||
|---|---|---|---|---|
| . | . | UV Irradiation . | . | |
| . | . | − . | + . | . |
| Experiment I | ||||
| 8.5-MAMneo (−Dex) | − | 99 ± 0.2 | 42 ± 3.3 | |
| 8.5-MAMneo (+Dex) | − | 99 ± 0.2 | 44 ± 0.3 | |
| 7.5-MAMmcl-1 (−Dex) | − | 99 ± 0.7 | 38 ± 0.6 | |
| 7.5-MAMmcl-1 (+Dex) | + | 99 ± 0.3 | 76 ± 3.1 | |
| Experiment II | ||||
| V1-CMVneo | − | 99 ± 0.4 | 13 ± 3.1 | |
| V2-CMVneo | − | 99 ± 0.1 | 8 ± 2.8 | |
| S3-CMVmcl-1 | + | 98 ± 0.7 | 60 ± 4.8 | |
| S8-CMVmcl-1 | + | 98 ± 0.7 | 44 ± 7.6 | |
| Transfectant Clone . | Expression of mcl-1* . | % Viable Cells . | ||
|---|---|---|---|---|
| . | . | UV Irradiation . | . | |
| . | . | − . | + . | . |
| Experiment I | ||||
| 8.5-MAMneo (−Dex) | − | 99 ± 0.2 | 42 ± 3.3 | |
| 8.5-MAMneo (+Dex) | − | 99 ± 0.2 | 44 ± 0.3 | |
| 7.5-MAMmcl-1 (−Dex) | − | 99 ± 0.7 | 38 ± 0.6 | |
| 7.5-MAMmcl-1 (+Dex) | + | 99 ± 0.3 | 76 ± 3.1 | |
| Experiment II | ||||
| V1-CMVneo | − | 99 ± 0.4 | 13 ± 3.1 | |
| V2-CMVneo | − | 99 ± 0.1 | 8 ± 2.8 | |
| S3-CMVmcl-1 | + | 98 ± 0.7 | 60 ± 4.8 | |
| S8-CMVmcl-1 | + | 98 ± 0.7 | 44 ± 7.6 | |
In experiment I, the indicated transfectant clones (5 × 105 cells/mL) were exposed (+Dex) or not exposed (−Dex) to dexamethasone for 2 hours and then either exposed to UV irradiation for 2.5 minutes or not exposed to radiation. In experiment II, transfectants constitutively expressing mcl-1 (S3-CMVmcl-1 and S8-CMVmcl-1) and vector-only clones (V1-CMVneo and V2-CMVneo) were exposed (or not exposed) to UV irradiation for 3 minutes. Cell viability was assayed after 1 day, using the acridine orange/ethidium bromide method. The values shown are the mean ± SD of three independent experiments.
See Fig 1.