Effects of IL-6 and TNF- α on IL-4 and IFN- γ Production
| Stimulant7-150 . | IL-47-151 . | IFN-γ7-151 . | Mast Cells/ . | Histamine/Culture . |
|---|---|---|---|---|
| (10 ng/mL) . | (pg/mL) . | (pg/mL) . | Culture . | (μg ± SD) . |
| . | . | . | (104 ± SD) . | . |
| Experiment 1 | ||||
| None | 174 ± 6 | 290 ± 22 | 2.9 ± 0.9 | 0.0410 ± 0.0104 |
| rIL-6 | 96 ± 77-152 | 411 ± 31‡ | 26.2 ± 2.0 | 0.2732 ± 0.0685ρ |
| Experiment 2 | ||||
| None | 199 ± 6 | 259 ± 20 | 4.2 ± 1.6 | 0.0509 ± 0.0034 |
| rTNF-α | 25 ± 2ρ | 1905 ± 131ρ | 77.5 ± 9.7 | 0.3887 ± 0.0335ρ |
| Stimulant7-150 . | IL-47-151 . | IFN-γ7-151 . | Mast Cells/ . | Histamine/Culture . |
|---|---|---|---|---|
| (10 ng/mL) . | (pg/mL) . | (pg/mL) . | Culture . | (μg ± SD) . |
| . | . | . | (104 ± SD) . | . |
| Experiment 1 | ||||
| None | 174 ± 6 | 290 ± 22 | 2.9 ± 0.9 | 0.0410 ± 0.0104 |
| rIL-6 | 96 ± 77-152 | 411 ± 31‡ | 26.2 ± 2.0 | 0.2732 ± 0.0685ρ |
| Experiment 2 | ||||
| None | 199 ± 6 | 259 ± 20 | 4.2 ± 1.6 | 0.0509 ± 0.0034 |
| rTNF-α | 25 ± 2ρ | 1905 ± 131ρ | 77.5 ± 9.7 | 0.3887 ± 0.0335ρ |
Mouse spleen cells were cultured with complete culture medium in the presence of either rIL-6 or rTNF-α. Mast cell numbers and histamine quantities were determined on day 12.
IFN-γ and IL-4 in the supernatants recovered on day 2 (for IFN-γ) and day 4 (for IL-4) from the cultures were detected by ELISA.
P < .05 compared with data without stimulants.
ρ P < .01 compared with data without stimulants.