Table 6. Effect of Macrophages on Mast... | American Society of Hematology

Table 6.

Effect of Macrophages on Mast Cell Development Induced by IL-6, TNF-α, and LPS

Stimulant (10 ng/mL)	Without Macrophages^6-150		With Macrophages
	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)
None	1.6 ± 0.6	0.0182 ± 0.0027	11.6 ± 3.8^6-151	0.0725 ± 0.0351
rIL-6	40.5 ± 11.4	0.1878 ± 0.0241	78.7 ± 16.7^6-151	0.5000 ± 0.0662^6-152
rTNF-α	59.5 ± 2.9	0.1249 ± 0.0263	85.0 ± 8.7^6-151	0.2054 ± 0.0145^6-151
LPS	34.1 ± 16.0	0.0570 ± 0.0435	94.1 ± 12.9^6-151	0.4378 ± 0.1211^6-151

Stimulant (10 ng/mL)	Without Macrophages^6-150		With Macrophages
	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)
None	1.6 ± 0.6	0.0182 ± 0.0027	11.6 ± 3.8^6-151	0.0725 ± 0.0351
rIL-6	40.5 ± 11.4	0.1878 ± 0.0241	78.7 ± 16.7^6-151	0.5000 ± 0.0662^6-152
rTNF-α	59.5 ± 2.9	0.1249 ± 0.0263	85.0 ± 8.7^6-151	0.2054 ± 0.0145^6-151
LPS	34.1 ± 16.0	0.0570 ± 0.0435	94.1 ± 12.9^6-151	0.4378 ± 0.1211^6-151

Macrophages were removed by Sephadex G-10 columns. Mast cell number and histamine quantity per culture were determined on day 12.

P < .05 compared with data of macrophage-depleted cultures.

P < .01 compared with data of macrophage-depleted cultures.