Table 5. Failure to Suppress Mast Cell... | American Society of Hematology

Table 5.

Failure to Suppress Mast Cell Development by Depleting Endogenous IL-6 and TNF-α With Neutralizing Antibody

Stimulant (10 ng/mL)	Antibody^5-150	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)
	(3 μg/mL)
None	−^5-151	2.5 ± 1.0	0.0112 ± 0.0005
rIFN-γ	−	66.6 ± 4.2	0.2088 ± 0.0322
	Anti–IFN-γ	1.6 ± 0.6	0.0116 ± 0.0005^5-152
	Anti–IL-6	68.3 ± 2.3	0.2147 ± 0.0032
	Anti–TNF-α	60.8 ± 5.6	0.1931 ± 0.0118
PGE1	−	102.8 ± 16.2	0.2471 ± 0.0321
	Anti–IL-6	79.8 ± 16.9	0.2346 ± 0.0502
	Anti–TNF-α	88.9 ± 1.2	0.2693 ± 0.0157
rTNF-α	−	102.9 ± 3.6	0.3156 ± 0.0193
	Anti–IL-6	109.3 ± 20.5	0.3977 ± 0.0567
	Anti–TNF-α	5.4 ± 2.6	0.0180 ± 0.0019^5-152
rIL-6	−	80.0 ± 7.1	0.4373 ± 0.0909
	Anti–IL-6	18.3 ± 5.0	0.1272 ± 0.0216^5-152
	Anti–TNF-α	70.4 ± 11.4	0.3899 ± 0.0381

Stimulant (10 ng/mL)	Antibody^5-150	Mast Cells/Culture (10⁴ ± SD)	Histamine/Culture (μg ± SD)
	(3 μg/mL)
None	−^5-151	2.5 ± 1.0	0.0112 ± 0.0005
rIFN-γ	−	66.6 ± 4.2	0.2088 ± 0.0322
	Anti–IFN-γ	1.6 ± 0.6	0.0116 ± 0.0005^5-152
	Anti–IL-6	68.3 ± 2.3	0.2147 ± 0.0032
	Anti–TNF-α	60.8 ± 5.6	0.1931 ± 0.0118
PGE1	−	102.8 ± 16.2	0.2471 ± 0.0321
	Anti–IL-6	79.8 ± 16.9	0.2346 ± 0.0502
	Anti–TNF-α	88.9 ± 1.2	0.2693 ± 0.0157
rTNF-α	−	102.9 ± 3.6	0.3156 ± 0.0193
	Anti–IL-6	109.3 ± 20.5	0.3977 ± 0.0567
	Anti–TNF-α	5.4 ± 2.6	0.0180 ± 0.0019^5-152
rIL-6	−	80.0 ± 7.1	0.4373 ± 0.0909
	Anti–IL-6	18.3 ± 5.0	0.1272 ± 0.0216^5-152
	Anti–TNF-α	70.4 ± 11.4	0.3899 ± 0.0381

F5-150

Various stimulants and neutralizing antibody were added at the start of the cultures. Mast cell numbers and histamine quantities per culture were determined on day 12.

F5-151

−, not added.

F5-152

P < .01 compared with data without neutralizing antibody.

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