Effects of IL-6, IL-9, SCF, or TNF- α on Colony Formation of Purified MMC in the Presence of rIL-3
| Addition . | MCC/Culture4-150 . |
|---|---|
| rIL-3 (20 ng/mL) | 19 ± 2 |
| rIL-3 + rIL-6 (20 ng/mL) | 20 ± 3 |
| rIL-3 + rIL-9 (40 ng/mL) | 27 ± 5 |
| rIL-3 + rSCF (40 ng/mL) | 33 ± 44-151 |
| rIL-3 + rTNF-α (40 ng/mL) | 17 ± 1 |
| Addition . | MCC/Culture4-150 . |
|---|---|
| rIL-3 (20 ng/mL) | 19 ± 2 |
| rIL-3 + rIL-6 (20 ng/mL) | 20 ± 3 |
| rIL-3 + rIL-9 (40 ng/mL) | 27 ± 5 |
| rIL-3 + rSCF (40 ng/mL) | 33 ± 44-151 |
| rIL-3 + rTNF-α (40 ng/mL) | 17 ± 1 |
Abbreviation: MCC, mast cell colonies.
Mast cells (1 × 103 cells/mL), induced by a 16-day liquid culture of spleen cells with 1 ng/mL LPS and purified by Histopaque-1077 separation, were cultured by the methylcellulose method in the presence of rIL-3. MCC in triplicate cultures were determined on day 16, as described in Materials and Methods.
P < .05 compared with data of rIL-3 alone.