Mast Cell Development From Bone Marrow Cells in a Liquid Culture
| Stimulant . | Mast Cells/Culture3-150 (104 ± SD) . | Histamine/Culture (μg ± SD) . | Endogenous . |
|---|---|---|---|
| (10 ng/mL) . | . | . | IL-3 in Supernatants3-151 . |
| None | 0 | 0 | Undetectable |
| PGE | 0 | 0 | Undetectable |
| rIFN-γ | 0 | 0 | Undetectable |
| rTNF-α | 0 | 0 | Undetectable |
| rIL-6 | 0 | 0 | Undetectable |
| rIL-3 | 40.8 ± 3.8 | 0.2051 ± 0.0034 | |
| rIL-3 + PGE | 163.3 ± 10.2 | 0.4059 ± 0.03133-152 | |
| rIL-3 + rINF-γ | 100.4 ± 16.2 | 0.5185 ± 0.04013-152 | |
| rIL-3 + rTNF-α | 73.3 ± 9.2 | 0.2317 ± 0.01353-152 | |
| rIL-3 ± rIL-6 | 146.2 ± 51.1 | 0.5106 ± 0.04723-152 |
| Stimulant . | Mast Cells/Culture3-150 (104 ± SD) . | Histamine/Culture (μg ± SD) . | Endogenous . |
|---|---|---|---|
| (10 ng/mL) . | . | . | IL-3 in Supernatants3-151 . |
| None | 0 | 0 | Undetectable |
| PGE | 0 | 0 | Undetectable |
| rIFN-γ | 0 | 0 | Undetectable |
| rTNF-α | 0 | 0 | Undetectable |
| rIL-6 | 0 | 0 | Undetectable |
| rIL-3 | 40.8 ± 3.8 | 0.2051 ± 0.0034 | |
| rIL-3 + PGE | 163.3 ± 10.2 | 0.4059 ± 0.03133-152 | |
| rIL-3 + rINF-γ | 100.4 ± 16.2 | 0.5185 ± 0.04013-152 | |
| rIL-3 + rTNF-α | 73.3 ± 9.2 | 0.2317 ± 0.01353-152 | |
| rIL-3 ± rIL-6 | 146.2 ± 51.1 | 0.5106 ± 0.04723-152 |
Mouse bone marrow cells were cultured with complete culture medium. Various cytokines were added at the start of the cultures. Considering the lack of endogenous IL-3 and the property of IL-3–dependent manner of MMC, rIL-3 was replenished at each feeding. Mast cell number and histamine quantity per culture were determined on day 12.
Endogenous IL-3 in the supernatants recovered from the cultures on days 2 and 4 was detected by ELISA.
P < .01 compared with data of rIL-3 alone.