Effects of Various Cytokines on Mast Cell Development
| Cytokine (per mL) . | Mast Cells/Culture (104 ± SD) . | Histamine/Culture (μg ± SD) . |
|---|---|---|
| None | 3.7 ± 1.0 | 0.0491 ± 0.0140 |
| rGM-CSF 10 ng | 0.2 ± 0.2 | 0.0232 ± 0.0017 |
| 1 ng | 0.5 ± 0.4 | 0.0321 ± 0.0027 |
| rIL-1β 20 ng | 1.9 ± 0.3 | 0.0326 ± 0.0016 |
| 10 ng | 2.7 ± 0.5 | 0.0373 ± 0.0016 |
| 1 ng | 2.0 ± 0.9 | 0.0285 ± 0.0057 |
| rIL-3 20 ng | 19.1 ± 8.5 | 0.1578 ± 0.0076 |
| 10 ng | 13.1 ± 4.4 | 0.1408 ± 0.0331 |
| 1 ng | 12.1 ± 2.9 | 0.1172 ± 0.0052 |
| rIL-5 20 U | 5.8 ± 1.5 | 0.0668 ± 0.0054 |
| 10 U | 3.7 ± 2.0 | 0.0427 ± 0.0052 |
| 5 U | 6.6 ± 2.1 | 0.0555 ± 0.0218 |
| rIL-6 20 ng | 111.6 ± 39.9 | 0.5138 ± 0.0154 |
| 10 ng | 111.2 ± 4.7 | 0.5789 ± 0.0180 |
| 1 ng | 59.1 ± 11.9 | 0.2692 ± 0.0129 |
| 0.1 ng | 6.6 ± 1.5 | 0.0203 ± 0.0038 |
| rIL-9 20 ng | 5.8 ± 0.6 | 0.0531 ± 0.0052 |
| 10 ng | 5.0 ± 1.0 | 0.0624 ± 0.0070 |
| 1 ng | 7.9 ± 2.1 | 0.0732 ± 0.0163 |
| rSCF 20 ng | 9.1 ± 4.6 | 0.0737 ± 0.0044 |
| 10 ng | 5.0 ± 1.2 | 0.0671 ± 0.0073 |
| 1 ng | 6.2 ± 1.2 | 0.0420 ± 0.0096 |
| rTGF-β1 10 ng | 4.5 ± 1.4 | 0.0196 ± 0.0018 |
| 1 ng | 1.1 ± 0.2 | 0.0178 ± 0.0043 |
| rTNF-α 20 ng | 166.2 ± 12.8 | 0.2989 ± 0.0101 |
| 10 ng | 192.9 ± 26.2 | 0.3584 ± 0.0368 |
| 1 ng | 5.0 ± 2.7 | 0.0296 ± 0.0022 |
| Cytokine (per mL) . | Mast Cells/Culture (104 ± SD) . | Histamine/Culture (μg ± SD) . |
|---|---|---|
| None | 3.7 ± 1.0 | 0.0491 ± 0.0140 |
| rGM-CSF 10 ng | 0.2 ± 0.2 | 0.0232 ± 0.0017 |
| 1 ng | 0.5 ± 0.4 | 0.0321 ± 0.0027 |
| rIL-1β 20 ng | 1.9 ± 0.3 | 0.0326 ± 0.0016 |
| 10 ng | 2.7 ± 0.5 | 0.0373 ± 0.0016 |
| 1 ng | 2.0 ± 0.9 | 0.0285 ± 0.0057 |
| rIL-3 20 ng | 19.1 ± 8.5 | 0.1578 ± 0.0076 |
| 10 ng | 13.1 ± 4.4 | 0.1408 ± 0.0331 |
| 1 ng | 12.1 ± 2.9 | 0.1172 ± 0.0052 |
| rIL-5 20 U | 5.8 ± 1.5 | 0.0668 ± 0.0054 |
| 10 U | 3.7 ± 2.0 | 0.0427 ± 0.0052 |
| 5 U | 6.6 ± 2.1 | 0.0555 ± 0.0218 |
| rIL-6 20 ng | 111.6 ± 39.9 | 0.5138 ± 0.0154 |
| 10 ng | 111.2 ± 4.7 | 0.5789 ± 0.0180 |
| 1 ng | 59.1 ± 11.9 | 0.2692 ± 0.0129 |
| 0.1 ng | 6.6 ± 1.5 | 0.0203 ± 0.0038 |
| rIL-9 20 ng | 5.8 ± 0.6 | 0.0531 ± 0.0052 |
| 10 ng | 5.0 ± 1.0 | 0.0624 ± 0.0070 |
| 1 ng | 7.9 ± 2.1 | 0.0732 ± 0.0163 |
| rSCF 20 ng | 9.1 ± 4.6 | 0.0737 ± 0.0044 |
| 10 ng | 5.0 ± 1.2 | 0.0671 ± 0.0073 |
| 1 ng | 6.2 ± 1.2 | 0.0420 ± 0.0096 |
| rTGF-β1 10 ng | 4.5 ± 1.4 | 0.0196 ± 0.0018 |
| 1 ng | 1.1 ± 0.2 | 0.0178 ± 0.0043 |
| rTNF-α 20 ng | 166.2 ± 12.8 | 0.2989 ± 0.0101 |
| 10 ng | 192.9 ± 26.2 | 0.3584 ± 0.0368 |
| 1 ng | 5.0 ± 2.7 | 0.0296 ± 0.0022 |
Mouse spleen cells were cultured with complete culture medium. Various cytokines were added at the start of the cultures. Mast cell number and histamine quantity per culture were determined on day 12.