Table 6. FISH Analysis of Sorted CD34... | American Society of Hematology

Table 6.

FISH Analysis of Sorted CD34⁺ Cells From Nonfrozen Maternal PB

Sample	Fetal Karyotype by CAAF	No. of Cells Sorted	No. of Cells Examined by FISH	No. of Cells With XY Signal	Hybridization Efficiency^6-150	Fetal Sex Correctly Assigned by
					X and Y	FACS/FISH
					Probe (%)
32	46,XY	2,527	32	0	38/78	N
33	46,XY	4,467	133	0	33/85	N
35	46,XY	6,496	588	2	53/84	Y
36	46,XY	4,350	186	42	88/92	Y
37	46,XY	1,695	169	1	78/86	Y
38	46,XY	4,589	455	0	76/78	N
40	46,XY	4,672	460	25	83/73	Y
34	46,XX	1,729	178	0	68/92	n/a
39	46,XX	3,248	345	3	89/78	N
41	46,XX	3,631	182	19	55/68	N

Sample	Fetal Karyotype by CAAF	No. of Cells Sorted	No. of Cells Examined by FISH	No. of Cells With XY Signal	Hybridization Efficiency^6-150	Fetal Sex Correctly Assigned by
					X and Y	FACS/FISH
					Probe (%)
32	46,XY	2,527	32	0	38/78	N
33	46,XY	4,467	133	0	33/85	N
35	46,XY	6,496	588	2	53/84	Y
36	46,XY	4,350	186	42	88/92	Y
37	46,XY	1,695	169	1	78/86	Y
38	46,XY	4,589	455	0	76/78	N
40	46,XY	4,672	460	25	83/73	Y
34	46,XX	1,729	178	0	68/92	n/a
39	46,XX	3,248	345	3	89/78	N
41	46,XX	3,631	182	19	55/68	N

PB (n = 10) was drawn at 10-13 weeks' gestation before any invasive diagnostic procedure and the FISH was performed without previous knowledge of the fetal karyotype. After sorting, cells were cultured for 18 hours in serum-free media Epo, MGF, IL-6, GM-CSF/IL-3 fusion protein, M-CSF, and Flt-3 ligand (samples 40 and 41 only) and the results from both cultures were pooled.

Abbreviations: n/a; not applicable; CAAF, cytogenetic analysis of amniotic fluid.

F6-150

Hybridization efficiency was controlled in each experiment using pure male and female mononuclear cells.

View Large

This Feature Is Available To Subscribers Only