Table 5. FISH Analysis of Sorted... | American Society of Hematology

Table 5.

FISH Analysis of Sorted Nucleated Erythroid Cells From Nonfrozen Maternal PB

Sample	Fetal Karyotype by CAAF	No. of Cells Sorted	No. of Cells Examined by FISH	No. of Cells With XY Signal	Hybridization Efficiency^5-150	Fetal Sex Correctly
					X and Y	Assigned by FACS/FISH
					Probe (%)
32	46,XY	1.2 × 10⁶	160	1	57/80	Y
33	46,XY	1.1 × 10⁶	81	0	48/85	N
35	46,XY	1.7 × 10⁶	101	0	64/86	N
36	46,XY	3.7 × 10⁵	57	0	85/88	N
37	46,XY	1.7 × 10⁵	0	0	76/91	N
38	46,XY	5.3 × 10⁵	417	21	76/78	Y
40	46,XY	7.0 × 10⁵	385	68	83/73	Y
34	46,XX	5.8 × 10⁵	9	0	78/86	n/a
39	46,XX	1.2 × 10⁶	174	2	89/78	N
41	46,XX	2.7 × 10⁵	136	0	68/68	n/a

Sample	Fetal Karyotype by CAAF	No. of Cells Sorted	No. of Cells Examined by FISH	No. of Cells With XY Signal	Hybridization Efficiency^5-150	Fetal Sex Correctly
					X and Y	Assigned by FACS/FISH
					Probe (%)
32	46,XY	1.2 × 10⁶	160	1	57/80	Y
33	46,XY	1.1 × 10⁶	81	0	48/85	N
35	46,XY	1.7 × 10⁶	101	0	64/86	N
36	46,XY	3.7 × 10⁵	57	0	85/88	N
37	46,XY	1.7 × 10⁵	0	0	76/91	N
38	46,XY	5.3 × 10⁵	417	21	76/78	Y
40	46,XY	7.0 × 10⁵	385	68	83/73	Y
34	46,XX	5.8 × 10⁵	9	0	78/86	n/a
39	46,XX	1.2 × 10⁶	174	2	89/78	N
41	46,XX	2.7 × 10⁵	136	0	68/68	n/a

PB (n = 10) was drawn at 10-13 weeks' gestation before any invasive diagnostic procedure and the FISH was performed without previous knowledge of the fetal karyotype. After sorting, cells were cultured for either 18 hours or 5 days in serum-free media Epo, MGF, IL-6, GM-CSF/IL-3 fusion protein, M-CSF, and Flt-3 ligand (samples 40 and 41 only) and the results from both cultures were pooled.

Abbreviations: n/a; not applicable; CAAF, cytogenetic analysis of amniotic fluid.

F5-150

Hybridization efficiency was controlled in each experiment using pure male and female mononuclear cells.

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