p53, p21, and Ki-67 Immunohistochemical Expression (IHC) and p53 Gene Status in NHLs
| Case No. . | Histologic Diagnosis . | p53 . | p21 . | Ki-67 . | ||
|---|---|---|---|---|---|---|
| . | W.F. Classification . | REAL Classification . | IHC (%) . | Mutation . | IHC (%) . | IHC (%) . |
| N124 | NHL-J | Burkitt's | 65 | cod.286, G→A, Glu→Lys | — | 90 |
| N311 | NHL-J | Burkitt's | 20 | cod.132, A→C, Lys→Gln | — | 90 |
| N312 | NHL-J | Burkitt's | 80 | cod.130, C→G, Leu→Val | — | 90 |
| N313 | NHL-J | Burkitt's | 30 | cod.132, A→C, Lys→Gln | — | NE |
| N315 | NHL-J | Burkitt's | 80 | cod.273, G→T, Arg→Leu | 80 | 90 |
| N519 | NHL-J | Burkitt's | 90 | cod.236, T→C, Tyr→His | 90 | 90 |
| N523 | NHL-J | Burkitt's | 10 | cod.267, G→C, Arg→Pro | — | 70 |
| N521 | NHL-H | Diffuse large B cell | 50 | cod.244, G→A, Gly→Asp | — | 70 |
| L802 | NHL-G | Diffuse large B cell | 50 | cod.141, G→A, Cys→Tyr | — | 50 |
| N611 | NHL-C | Follicular center (grade II) | 20 | cod.161, G→A, Ala→Thr | — | 30 |
| N612 | NHL-C | Follicular center (grade II) | 60 | cod.248, G→A, Arg→Glu | — | 60 |
| L711 | NHL-A | Small lymphocytic | 40 | cod.193, A→G, His→Arg | — | 50 |
| L914 | NHL-A | Small lymphocytic | 60 | cod.258, G→A, Glu→Lys | — | 60 |
| N610 | NHL-A | Small lymphocytic | 60 | cod.275, G→A, Cys→Thr | — | 60 |
| F11 | NHL-H | Diffuse large B cell | 80 | cod.179, A→G, His→Arg | — | 70 |
| F12 | NHL-H | Diffuse large B cell | 80 | cod.282, C→T, Arg→Trp | — | 70 |
| F13 | NHL-G | Diffuse large B cell | 80 | cod.248, C→T, Arg→Trp | — | 60 |
| F15 | NHL-G | Diffuse large B cell | 80 | cod.285, G→A, Glu→Lys | — | 50 |
| N167 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | 30 | cod.175, G→A, Arg→His | — | 40 |
| N240 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 30 | cod.176, T→A, Cys→Ser | 10 | 30 |
| VR | NHL-Misc Intestinal T-cell | Intestinal T cell | 80 | NO | 50 | 50 |
| F33H | NHL-G | Diffuse large B cell | 30 | NO | 40 | 40 |
| N609 | NHL-A | Small lymphocytic | 50 | NO | 10 | 50 |
| N522 | NHL-G | Diffuse large B cell | 60 | NO | 10 | 70 |
| N615 | NHL-G | Diffuse large B cell | 40 | NO | 10 | 80 |
| F8 | NHL-H | Diffuse large B cell | 20 | NO | 25 | 45 |
| F3 | NHL-F | Angioimmunoblastic T cell | 15 | NO | 10 | 25 |
| F14 | NHL-H | Diffuse large B cell | 20 | NO | 15 | 50 |
| F10 | NHL-G | Diffuse large B cell | 15 | NO | 15 | 30 |
| F2 | NHL-G | Diffuse large B cell | 10 | NO | 15 | 45 |
| F4 | NHL-G | Diffuse large B cell | 10 | NO | — | 60 |
| F9 | NHL-G | Diffuse large B cell | 15 | NO | — | 70 |
| N32 | NHL-J | Burkitt's | 50 | NO | — | 80 |
| L985 | NHL-G | Diffuse large B cell | 10 | NO | — | 50 |
| N72 | NHL-H | Diffuse large B cell | 10 | NO | — | 70 |
| N614 | NHL-G | Diffuse large B cell | 10 | NO | — | 30 |
| N226 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 10 | NO | — | 20 |
| N227 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 10 | NO | — | ND |
| N169 | NHL-J | Burkitt's | 10 | NO | — | 80 |
| N318 | NHL-F | Diffuse large B cell | — | NO | — | 50 |
| N267 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | — | NO | 10 | 30 |
| N268 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | — | NO | 10 | 70 |
| N520 | NHL-G | Diffuse large B cell | — | NO | — | 80 |
| N503 | NHL-C | Follicular center (grade II) | — | NO | — | 50 |
| N613 | NHL-Misc MALT-type | Extranodal MZBC | — | NO | — | 20 |
| N319 | NHL-G | Diffuse large B cell | — | NO | — | 70 |
| F5 | NHL-G | Diffuse large B cell | — | NO | — | ND |
| F1 | NHL-Misc Monocytoid | Nodal MZBC | — | NO | 10 | 10 |
| F6 | NHL-B | Follicular center (grade I) | — | NO | — | 10 |
| F7 | NHL-B | Follicular center (grade I) | — | NO | — | ND |
| F33L | NHL-B | Follicular center (grade I) | — | NO | — | 10 |
| F16 | NHL-B | Follicular center (grade I) | — | ND | — | ND |
| F17 | NHL-G | Diffuse large B cell | — | ND | — | 60 |
| F18 | NHL-G | Diffuse large B cell | — | ND | — | 35 |
| F19 | NHL-D | Follicular center (grade III) | — | ND | — | 5 |
| F20 | NHL-B | Follicular center (grade I) | — | ND | — | ND |
| F21 | NHL-B | Follicular center (grade I) | — | ND | — | 15 |
| F22 | NHL-G | Diffuse large B cell | — | ND | — | 35 |
| F23 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F24 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F25 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F26 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 10 |
| F27 | NHL-H | Diffuse large B cell | 50 | ND | 20 | 70 |
| F28 | NHL-G | Diffuse large B cell | 10 | ND | 10 | 40 |
| F29 | NHL-G | Diffuse large B cell | 30 | ND | 10 | 45 |
| F30 | NHL-G | Diffuse large B cell | 10 | ND | — | 60 |
| F31 | NHL-G | Diffuse large B cell | 10 | ND | — | ND |
| F32 | NHL-G | Diffuse large B cell | 15 | ND | — | 55 |
| F34 | NHL-H | Diffuse large B cell | 20 | ND | — | 35 |
| F35 | NHL-G | Diffuse large B cell | 20 | ND | — | 20 |
| Case No. . | Histologic Diagnosis . | p53 . | p21 . | Ki-67 . | ||
|---|---|---|---|---|---|---|
| . | W.F. Classification . | REAL Classification . | IHC (%) . | Mutation . | IHC (%) . | IHC (%) . |
| N124 | NHL-J | Burkitt's | 65 | cod.286, G→A, Glu→Lys | — | 90 |
| N311 | NHL-J | Burkitt's | 20 | cod.132, A→C, Lys→Gln | — | 90 |
| N312 | NHL-J | Burkitt's | 80 | cod.130, C→G, Leu→Val | — | 90 |
| N313 | NHL-J | Burkitt's | 30 | cod.132, A→C, Lys→Gln | — | NE |
| N315 | NHL-J | Burkitt's | 80 | cod.273, G→T, Arg→Leu | 80 | 90 |
| N519 | NHL-J | Burkitt's | 90 | cod.236, T→C, Tyr→His | 90 | 90 |
| N523 | NHL-J | Burkitt's | 10 | cod.267, G→C, Arg→Pro | — | 70 |
| N521 | NHL-H | Diffuse large B cell | 50 | cod.244, G→A, Gly→Asp | — | 70 |
| L802 | NHL-G | Diffuse large B cell | 50 | cod.141, G→A, Cys→Tyr | — | 50 |
| N611 | NHL-C | Follicular center (grade II) | 20 | cod.161, G→A, Ala→Thr | — | 30 |
| N612 | NHL-C | Follicular center (grade II) | 60 | cod.248, G→A, Arg→Glu | — | 60 |
| L711 | NHL-A | Small lymphocytic | 40 | cod.193, A→G, His→Arg | — | 50 |
| L914 | NHL-A | Small lymphocytic | 60 | cod.258, G→A, Glu→Lys | — | 60 |
| N610 | NHL-A | Small lymphocytic | 60 | cod.275, G→A, Cys→Thr | — | 60 |
| F11 | NHL-H | Diffuse large B cell | 80 | cod.179, A→G, His→Arg | — | 70 |
| F12 | NHL-H | Diffuse large B cell | 80 | cod.282, C→T, Arg→Trp | — | 70 |
| F13 | NHL-G | Diffuse large B cell | 80 | cod.248, C→T, Arg→Trp | — | 60 |
| F15 | NHL-G | Diffuse large B cell | 80 | cod.285, G→A, Glu→Lys | — | 50 |
| N167 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | 30 | cod.175, G→A, Arg→His | — | 40 |
| N240 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 30 | cod.176, T→A, Cys→Ser | 10 | 30 |
| VR | NHL-Misc Intestinal T-cell | Intestinal T cell | 80 | NO | 50 | 50 |
| F33H | NHL-G | Diffuse large B cell | 30 | NO | 40 | 40 |
| N609 | NHL-A | Small lymphocytic | 50 | NO | 10 | 50 |
| N522 | NHL-G | Diffuse large B cell | 60 | NO | 10 | 70 |
| N615 | NHL-G | Diffuse large B cell | 40 | NO | 10 | 80 |
| F8 | NHL-H | Diffuse large B cell | 20 | NO | 25 | 45 |
| F3 | NHL-F | Angioimmunoblastic T cell | 15 | NO | 10 | 25 |
| F14 | NHL-H | Diffuse large B cell | 20 | NO | 15 | 50 |
| F10 | NHL-G | Diffuse large B cell | 15 | NO | 15 | 30 |
| F2 | NHL-G | Diffuse large B cell | 10 | NO | 15 | 45 |
| F4 | NHL-G | Diffuse large B cell | 10 | NO | — | 60 |
| F9 | NHL-G | Diffuse large B cell | 15 | NO | — | 70 |
| N32 | NHL-J | Burkitt's | 50 | NO | — | 80 |
| L985 | NHL-G | Diffuse large B cell | 10 | NO | — | 50 |
| N72 | NHL-H | Diffuse large B cell | 10 | NO | — | 70 |
| N614 | NHL-G | Diffuse large B cell | 10 | NO | — | 30 |
| N226 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 10 | NO | — | 20 |
| N227 | NHL-Misc ALC (Ki-1+) | Anaplastic large cell | 10 | NO | — | ND |
| N169 | NHL-J | Burkitt's | 10 | NO | — | 80 |
| N318 | NHL-F | Diffuse large B cell | — | NO | — | 50 |
| N267 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | — | NO | 10 | 30 |
| N268 | NHL-Misc ALC (Ki-1+) | Diffuse large B cell | — | NO | 10 | 70 |
| N520 | NHL-G | Diffuse large B cell | — | NO | — | 80 |
| N503 | NHL-C | Follicular center (grade II) | — | NO | — | 50 |
| N613 | NHL-Misc MALT-type | Extranodal MZBC | — | NO | — | 20 |
| N319 | NHL-G | Diffuse large B cell | — | NO | — | 70 |
| F5 | NHL-G | Diffuse large B cell | — | NO | — | ND |
| F1 | NHL-Misc Monocytoid | Nodal MZBC | — | NO | 10 | 10 |
| F6 | NHL-B | Follicular center (grade I) | — | NO | — | 10 |
| F7 | NHL-B | Follicular center (grade I) | — | NO | — | ND |
| F33L | NHL-B | Follicular center (grade I) | — | NO | — | 10 |
| F16 | NHL-B | Follicular center (grade I) | — | ND | — | ND |
| F17 | NHL-G | Diffuse large B cell | — | ND | — | 60 |
| F18 | NHL-G | Diffuse large B cell | — | ND | — | 35 |
| F19 | NHL-D | Follicular center (grade III) | — | ND | — | 5 |
| F20 | NHL-B | Follicular center (grade I) | — | ND | — | ND |
| F21 | NHL-B | Follicular center (grade I) | — | ND | — | 15 |
| F22 | NHL-G | Diffuse large B cell | — | ND | — | 35 |
| F23 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F24 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F25 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 5 |
| F26 | NHL-Misc MALT-type | Extranodal MZBC | — | ND | — | 10 |
| F27 | NHL-H | Diffuse large B cell | 50 | ND | 20 | 70 |
| F28 | NHL-G | Diffuse large B cell | 10 | ND | 10 | 40 |
| F29 | NHL-G | Diffuse large B cell | 30 | ND | 10 | 45 |
| F30 | NHL-G | Diffuse large B cell | 10 | ND | — | 60 |
| F31 | NHL-G | Diffuse large B cell | 10 | ND | — | ND |
| F32 | NHL-G | Diffuse large B cell | 15 | ND | — | 55 |
| F34 | NHL-H | Diffuse large B cell | 20 | ND | — | 35 |
| F35 | NHL-G | Diffuse large B cell | 20 | ND | — | 20 |
Abbreviations: ALC, anaplastic large cell; MZBC, marginal zone B-cell; −, negative; NO: no mutation; ND, not done; NE, not evaluable.