Table 1.

Reconstitution and lyt2 Expression in the SCID-hu Bone Assay

BoneUBC Cells*% Donor% lyt2+ of Donor% MA2.1+lyt2+ρ% CD14+lyt2+1-155
No cells 86 
No cells 100 
RevM10/lyt2 69 42 29 11 
RevM10/lyt2 72 54 39 
RevM10/lyt2 38 50 19 
RevM10/lyt2 18 52 
RevM10/lyt2 61 45 28 
RevM10/lyt2 50 19 11 
RevM10/lyt2 81 16 13 14 
10 RevM10/lyt2 73 23 17 11 
BoneUBC Cells*% Donor% lyt2+ of Donor% MA2.1+lyt2+ρ% CD14+lyt2+1-155
No cells 86 
No cells 100 
RevM10/lyt2 69 42 29 11 
RevM10/lyt2 72 54 39 
RevM10/lyt2 38 50 19 
RevM10/lyt2 18 52 
RevM10/lyt2 61 45 28 
RevM10/lyt2 50 19 11 
RevM10/lyt2 81 16 13 14 
10 RevM10/lyt2 73 23 17 11 
*

CD34+ cells transduced with the retroviral vectors. No cells indicate irradiated bones with no injection.

% MA2.1+ cells from each reconstituted bone fragment. The no cell control shows staining background.

% lyt2+ cells of MA2.1+ cells indicating marking efficiency of the donor cells.

ρ % MA2.1+lyt2+ cells of total live, lymphoid, and myeloid blast cells.

F1-155

% CD14+lyt2+ cells of total live cells.

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