Reconstitution and lyt2 Expression in the SCID-hu Bone Assay
Bone . | UBC Cells* . | % Donor† . | % lyt2+ of Donor‡ . | % MA2.1+lyt2+ρ . | % CD14+lyt2+1-155 . |
---|---|---|---|---|---|
1 | No cells | 4 | 86 | 4 | 3 |
2 | No cells | 4 | 100 | 4 | 3 |
3 | RevM10/lyt2 | 69 | 42 | 29 | 11 |
4 | RevM10/lyt2 | 72 | 54 | 39 | 8 |
5 | RevM10/lyt2 | 38 | 50 | 19 | 6 |
6 | RevM10/lyt2 | 18 | 52 | 9 | 4 |
7 | RevM10/lyt2 | 61 | 45 | 28 | 9 |
8 | RevM10/lyt2 | 50 | 19 | 9 | 11 |
9 | RevM10/lyt2 | 81 | 16 | 13 | 14 |
10 | RevM10/lyt2 | 73 | 23 | 17 | 11 |
Bone . | UBC Cells* . | % Donor† . | % lyt2+ of Donor‡ . | % MA2.1+lyt2+ρ . | % CD14+lyt2+1-155 . |
---|---|---|---|---|---|
1 | No cells | 4 | 86 | 4 | 3 |
2 | No cells | 4 | 100 | 4 | 3 |
3 | RevM10/lyt2 | 69 | 42 | 29 | 11 |
4 | RevM10/lyt2 | 72 | 54 | 39 | 8 |
5 | RevM10/lyt2 | 38 | 50 | 19 | 6 |
6 | RevM10/lyt2 | 18 | 52 | 9 | 4 |
7 | RevM10/lyt2 | 61 | 45 | 28 | 9 |
8 | RevM10/lyt2 | 50 | 19 | 9 | 11 |
9 | RevM10/lyt2 | 81 | 16 | 13 | 14 |
10 | RevM10/lyt2 | 73 | 23 | 17 | 11 |
CD34+ cells transduced with the retroviral vectors. No cells indicate irradiated bones with no injection.
% MA2.1+ cells from each reconstituted bone fragment. The no cell control shows staining background.
% lyt2+ cells of MA2.1+ cells indicating marking efficiency of the donor cells.
ρ % MA2.1+lyt2+ cells of total live, lymphoid, and myeloid blast cells.
% CD14+lyt2+ cells of total live cells.