Effect of IL-3 on TPO-Induced Polyploidy in MK Cultures
| Concentration IL-3 (ng/mL) . | Percent MK With Ploidy ≥16N . | ||||||
|---|---|---|---|---|---|---|---|
| . | Concentration TPO (U/mL) . | . | . | . | |||
| . | 0 . | 0.75 . | 15.0 . | 300 . | . | . | . |
| 0 | 0 | 5.0 | 17.3 | 18.0 | |||
| 0.3 | 2.5 | 2.3 | 5.5 | 7.5 | |||
| 1.0 | 3.3 | 3.5 | 3.9 | 5.0 | |||
| Concentration IL-3 (ng/mL) . | Percent MK With Ploidy ≥16N . | ||||||
|---|---|---|---|---|---|---|---|
| . | Concentration TPO (U/mL) . | . | . | . | |||
| . | 0 . | 0.75 . | 15.0 . | 300 . | . | . | . |
| 0 | 0 | 5.0 | 17.3 | 18.0 | |||
| 0.3 | 2.5 | 2.3 | 5.5 | 7.5 | |||
| 1.0 | 3.3 | 3.5 | 3.9 | 5.0 | |||
All cultures were performed with isolated CD34+CD41+ cells analyzed after 7 days in culture as described in the text. In replicate analyses of cultured BM cells, the error in the determination of the proportion of cells whose DNA content equals or exceeds 16N is ±10%.