Increased Binding of Transferrin by K562 Cells in Reponse to Epo
| Treatment . | Transferrin Binding (ng/106 cells) . | |
|---|---|---|
| . | 24 h . | 72 h . |
| None (control) | 2.6 ± 0.4 | 2.7 ± 1.1 |
| Desferrioxamine (100 μmol/L) | 7.9 ± 1.4* | 14.2 ± 2.5 |
| Epo (5 U/mL) | 4.2 ± 1.4† | 5.3 ± 1.1* |
| Epo (50 U/mL) | 4.9 ± 1.4* | 7.4 ± 2.3* |
| Treatment . | Transferrin Binding (ng/106 cells) . | |
|---|---|---|
| . | 24 h . | 72 h . |
| None (control) | 2.6 ± 0.4 | 2.7 ± 1.1 |
| Desferrioxamine (100 μmol/L) | 7.9 ± 1.4* | 14.2 ± 2.5 |
| Epo (5 U/mL) | 4.2 ± 1.4† | 5.3 ± 1.1* |
| Epo (50 U/mL) | 4.9 ± 1.4* | 7.4 ± 2.3* |
Cells were preincubated for 24 and 72 hours with human recombinant erythropoietin (Epo), desferrioxamine, or left untreated (control); were washed; and were incubated on ice with 125I-transferrin (2 μg) for 30 minutes. After consecutive washing steps, cell-bound 125I-transferrin was determined. Means ± SD for six independent experiments are shown. Calculation of statistical differences in relation to the control were performed using the Student's t-test.
P < .01.
P < .05.