Table 3. Influence of γ-secretase... | American Society of Hematology

Table 3.

Influence of γ-secretase inhibition by DAPT on frequency of cell subsets in FTOC seeded with human CD34⁺CD1^- thymic progenitor cells after 13 and 19 days of culture

		DAPT treatment
Cell subset	Control	5 μM	10 μM
After 13 days
CD4ISP	57.3 ± 1.7	43.0 ± 2.6	35.3 ± 0.8
DP	14.7 ± 6.0	52.6 ± 5.4^*	56.7 ± 0.6^*
IC TCR-β	26.3 ± 7.1	1.3 ± 0.1^*	0.9 ± 0.2^*
CD3⁺	17.9 ± 4.6	1.0 ± 0.2^*	0.8 ± 0.2^*
TCR-αβ	1.3 ± 0.3	0.5 ± 0.1^*	0.3 ± 0.1^*
TCR-γδ	4.0 ± 0.5	0.2 ± 0.1^*	0.1 ± 0.0^*
After 19 days
CD56⁺	0.2 ± 0.1	2.7 ± 0.3^*	8.3 ± 2.1^*
CD4ISP	34.4 ± 6.3	16.6 ± 5.2^*	8.7 ± 2.0^*
DP	56.3 ± 9.3	68.9 ± 8.8^*	71.1 ± 3.6^*
IC TCR-β	63.4 ± 7.1	5.3 ± 1.2^*	2.1 ± 1.7^*
CD3⁺	50.6 ± 3.5	5.1 ± 0.5^*	5.1 ± 0.8^*
TCR-αβ	36.8 ± 3.8	2.3 ± 0.6^*	2.3 ± 0.5^*
TCR-γδ	3.3 ± 0.9	0.4 ± 0.1^*	0.9 ± 0.5^*

		DAPT treatment
Cell subset	Control	5 μM	10 μM
After 13 days
CD4ISP	57.3 ± 1.7	43.0 ± 2.6	35.3 ± 0.8
DP	14.7 ± 6.0	52.6 ± 5.4^*	56.7 ± 0.6^*
IC TCR-β	26.3 ± 7.1	1.3 ± 0.1^*	0.9 ± 0.2^*
CD3⁺	17.9 ± 4.6	1.0 ± 0.2^*	0.8 ± 0.2^*
TCR-αβ	1.3 ± 0.3	0.5 ± 0.1^*	0.3 ± 0.1^*
TCR-γδ	4.0 ± 0.5	0.2 ± 0.1^*	0.1 ± 0.0^*
After 19 days
CD56⁺	0.2 ± 0.1	2.7 ± 0.3^*	8.3 ± 2.1^*
CD4ISP	34.4 ± 6.3	16.6 ± 5.2^*	8.7 ± 2.0^*
DP	56.3 ± 9.3	68.9 ± 8.8^*	71.1 ± 3.6^*
IC TCR-β	63.4 ± 7.1	5.3 ± 1.2^*	2.1 ± 1.7^*
CD3⁺	50.6 ± 3.5	5.1 ± 0.5^*	5.1 ± 0.8^*
TCR-αβ	36.8 ± 3.8	2.3 ± 0.6^*	2.3 ± 0.5^*
TCR-γδ	3.3 ± 0.9	0.4 ± 0.1^*	0.9 ± 0.5^*

Results represent mean percentage ± SEM of 3 to 5 independent experiments.

Significant difference (P < .05; paired Student t test) compared with vehicle-treated control cultures

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