Table 2. Influence of γ-secretase... | American Society of Hematology

Table 2.

Influence of γ-secretase inhibition by DAPT on human cellularity of cell subsets in FTOC seeded with human CD34⁺CD1^- thymic progenitor cells after 13 and 19 days of culture

Days after treatment, cell type	Control, no. cells	DAPT treatment
Days after treatment, cell type	Control, no. cells			5 μM, no. cells	10 μM, no. cells
After 13 days
Human cells	61 873 ± 10 604^*	85 730 ± 25 356	92 214 ± 4 988
CD4ISP	36 481 ± 5 404	38 364 ± 11 521	43 343 ± 2 869
DP	12 618 ± 6 568	52 810 ± 16 178†	62 758 ± 3 316†
IC TCR-β	21 176 ± 8 391	891 ± 340†	623 ± 343†
CD3⁺	14 467 ± 5 176	723 ± 426†	596 ± 292†
TCR-αβ	9 571 ± 3 505	413 ± 267†	456 ± 271†
TCR-γδ	3 035 ± 893	140 ± 166†	71 ± 62†
After 19 days
Human cells	208 397 ± 48 574	44 868 ± 7 632†	18 731 ± 7 118†
CD56⁺	377 ± 72	1 408 ± 343†	1 285 ± 770
CD4ISP	62 544 ± 15 294	7 012 ± 2 060†	1 933 ± 365†
DP	130 330 ± 40 609	30 574 ± 6 641†	16 843 ± 3 806†
IC TCR-β	125 048 ± 30 095	2 291 ± 730†	689 ± 609†
CD3⁺	109 360 ± 528 583	2 301 ± 448†	1 128 ± 125†
TCR-αβ	89 242 ± 20 756	1 342 ± 263†	630 ± 92†
TCR-γδ	6 096 ± 1 365	214 ± 66†	192 ± 88†

Days after treatment, cell type	Control, no. cells	DAPT treatment
Days after treatment, cell type	Control, no. cells			5 μM, no. cells	10 μM, no. cells
After 13 days
Human cells	61 873 ± 10 604^*	85 730 ± 25 356	92 214 ± 4 988
CD4ISP	36 481 ± 5 404	38 364 ± 11 521	43 343 ± 2 869
DP	12 618 ± 6 568	52 810 ± 16 178†	62 758 ± 3 316†
IC TCR-β	21 176 ± 8 391	891 ± 340†	623 ± 343†
CD3⁺	14 467 ± 5 176	723 ± 426†	596 ± 292†
TCR-αβ	9 571 ± 3 505	413 ± 267†	456 ± 271†
TCR-γδ	3 035 ± 893	140 ± 166†	71 ± 62†
After 19 days
Human cells	208 397 ± 48 574	44 868 ± 7 632†	18 731 ± 7 118†
CD56⁺	377 ± 72	1 408 ± 343†	1 285 ± 770
CD4ISP	62 544 ± 15 294	7 012 ± 2 060†	1 933 ± 365†
DP	130 330 ± 40 609	30 574 ± 6 641†	16 843 ± 3 806†
IC TCR-β	125 048 ± 30 095	2 291 ± 730†	689 ± 609†
CD3⁺	109 360 ± 528 583	2 301 ± 448†	1 128 ± 125†
TCR-αβ	89 242 ± 20 756	1 342 ± 263†	630 ± 92†
TCR-γδ	6 096 ± 1 365	214 ± 66†	192 ± 88†

Results represent mean ± SEM of 3 to 5 independent experiments.

Absolute cell number

†

Significant difference (P < .05; paired Student t test) compared with vehicle-treated control cultures

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