Enumeration of splenic cDC and pDC populations
. | Spleen DC populations (× 106) . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
. | Wild type . | Nfkb1-/- . | C-Rel-/- . | Nfkb1-/- C-Rel-/- . | Nfkb1-/- C-Rel-/- BM chimeras . | ||||
| Plasmacytoid | 0.93 ± 0.11 | 0.31 ± 0.08 | 0.47 ± 0.02 | 0.15 ± 0.06 | 0.43 ± 0.05 | ||||
| Conventional | 2.61 ± 0.21 | 1.19 ± 0.18 | 2.56 ± 0.21 | 0.48 ± 0.03 | 1.93 ± 0.24 | ||||
| CD4+ | 1.28 ± 0.06 | 0.48 ± 0.06 | 1.49 ± 0.24 | 0.19 ± 0.01 | ND | ||||
| CD8+ | 0.50 ± 0.09 | 0.39 ± 0.07 | 0.45 ± 0.04 | 0.23 ± 0.08 | ND | ||||
| CD4-CD8- | 0.48 ± 0.03 | 0.36 ± 0.03 | 0.54 ± 0.08 | 0.08 ± 0.01 | ND | ||||
. | Spleen DC populations (× 106) . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
. | Wild type . | Nfkb1-/- . | C-Rel-/- . | Nfkb1-/- C-Rel-/- . | Nfkb1-/- C-Rel-/- BM chimeras . | ||||
| Plasmacytoid | 0.93 ± 0.11 | 0.31 ± 0.08 | 0.47 ± 0.02 | 0.15 ± 0.06 | 0.43 ± 0.05 | ||||
| Conventional | 2.61 ± 0.21 | 1.19 ± 0.18 | 2.56 ± 0.21 | 0.48 ± 0.03 | 1.93 ± 0.24 | ||||
| CD4+ | 1.28 ± 0.06 | 0.48 ± 0.06 | 1.49 ± 0.24 | 0.19 ± 0.01 | ND | ||||
| CD8+ | 0.50 ± 0.09 | 0.39 ± 0.07 | 0.45 ± 0.04 | 0.23 ± 0.08 | ND | ||||
| CD4-CD8- | 0.48 ± 0.03 | 0.36 ± 0.03 | 0.54 ± 0.08 | 0.08 ± 0.01 | ND | ||||
Numbers of purified cDCs (total and CD4+, CD8+, and CD4-CD8- subpopulations) and pDCs isolated from wild-type, Nfkb1-/-, C-Rel-/-, and Nfkb1-/- C-Rel-/- mice or irradiated C57BL6Ly5. 1 mice engrafted with Nfkb1-/- C-Rel-/- bone marrow were determined. The results shown are the range within either five independent experiments (for total pDCs and cDCs) or three independent experiments (for cDC subsets) or two independent experiments for the chimera results.
ND indicates not determined.