Mode and access rates of FMS ingress into cell compartments and chelation of labile iron
. | Chelator . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | DFO . | . | DFP . | . | ICL670 . | . | |||||
| Compartment . | D . | E . | D . | E . | D . | E . | |||||
| Cytosol | – | + | +++ | + | ++++ | + | |||||
| Endosomes | – | + | ++ | + | +++ | + | |||||
| Mitochondria | – | – | + | – | + | – | |||||
. | Chelator . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | DFO . | . | DFP . | . | ICL670 . | . | |||||
| Compartment . | D . | E . | D . | E . | D . | E . | |||||
| Cytosol | – | + | +++ | + | ++++ | + | |||||
| Endosomes | – | + | ++ | + | +++ | + | |||||
| Mitochondria | – | – | + | – | + | – | |||||
The various chelators used in this study, deferrioxamine (DFO), deferriprone (DFP), and ICL670, are compared in terms of their modes and relative rates of access into model cells as determined by their capacity to bind labile iron (complexed to fluorescent probes) or reduce its involvement in ROS production. D and E indicate the access mode into the cell by diffusion or endocytosis, respectively.
– and + through ++++ denote a semiquantitative scale of the indicated property.