Influence of MAPK inhibitors on TNFα-induced inhibition of hTERT in KG1 cells
Cells . | hTERT/PBGD . |
|---|---|
| KG1 | 100 |
| KG1 + TNFα | 49.9 ± 9.8† |
| KG1 + SP600125 | 108.1 ± 18.1 |
| KG1 + TNFα + SP600125 | 98.5 ± 23.1* |
| KG1 + SB203580 | 33.1 ± 4.5 |
| KG1 + TNFα + SB203580 | 34.2 ± 11.6 |
Cells . | hTERT/PBGD . |
|---|---|
| KG1 | 100 |
| KG1 + TNFα | 49.9 ± 9.8† |
| KG1 + SP600125 | 108.1 ± 18.1 |
| KG1 + TNFα + SP600125 | 98.5 ± 23.1* |
| KG1 + SB203580 | 33.1 ± 4.5 |
| KG1 + TNFα + SB203580 | 34.2 ± 11.6 |
KG1 cells were pretreated or not with SP600125 (20 μM) or SB203580 (1 μM) for 1 hour, then treated with TNFα (20 ng/mL) for 2 to 6 hours. hTERT transcripts were quantified as described in “Materials and methods.” hTERT/PBGD represents the ratio between hTERT and PBGD transcripts normalized to untreated control cells. Results are expressed as mean hTERT/PBGD values ± SDs from 3 independent experiments.
P = .014
P = .006