Influence of TNFα on hTERT gene expression in AML cells
Sample no. . | hTERT/PBGD-untreated cells . | hTERT/PBGD TNFα, 2 h . | hTERT/PBGD TNFα, 6 h . |
|---|---|---|---|
| 1 | 100 | ND | 60.0 |
| 2 | 100 | 16.1 | Undetectable |
| 3 | 100 | 73.1 | ND |
| 4 | 100 | 51.9 | Undetectable |
| 5 | 100 | ND | 4.4 |
| 6 | 100 | 77.7 | 35.0 |
| 7 | 100 | 107.9 | 57.0 |
| 8 | 100 | 112.0 | 70.6 |
| 9 | 100 | ND | 41.5 |
| 10 | 100 | 92.2 | 79.7 |
| KG1* | 100 | 49.4 ± 9.8† | 27.2 ± 2.1‡ |
Sample no. . | hTERT/PBGD-untreated cells . | hTERT/PBGD TNFα, 2 h . | hTERT/PBGD TNFα, 6 h . |
|---|---|---|---|
| 1 | 100 | ND | 60.0 |
| 2 | 100 | 16.1 | Undetectable |
| 3 | 100 | 73.1 | ND |
| 4 | 100 | 51.9 | Undetectable |
| 5 | 100 | ND | 4.4 |
| 6 | 100 | 77.7 | 35.0 |
| 7 | 100 | 107.9 | 57.0 |
| 8 | 100 | 112.0 | 70.6 |
| 9 | 100 | ND | 41.5 |
| 10 | 100 | 92.2 | 79.7 |
| KG1* | 100 | 49.4 ± 9.8† | 27.2 ± 2.1‡ |
Fresh AML cells and KG1 leukemia cell line were treated or not with TNFα (20 ng/mL) for 2 to 6 hours. hTERT transcripts were quantified as described in “Materials and methods.” Results are expressed as undetectable or as the ratio between hTERT and PBGD transcripts normalized to untreated cells.
ND indicates not done.
For KG1, results are the mean ± SEM of 10 independents experiments
P = .006
P > .001