The infiltrated monocytes/macrophages within the peritoneum do not undergo apoptosis
. | Percentage of total cells ± SD . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 4 d after TG injection . | . | . | 6 d after TG injection . | . | 8 d after TG injection . | . | ||||||
| . | 0 h in vitro culture . | 20 h in vitro culture . | . | 0 h in vitro culture . | 20 h in vitro culture . | . | 20 h in vitro culture . | ||||||
. | . | –LPS/SB . | +LPS/SB . | . | . | 0 h in vitro culture . | . | ||||||
| WT | 2.3 ± 0.13 | 6.7 ± 0.56 | 46.9 ± 1.7 | 1.36 ± 1.1 | 1.43 ± 0.79 | 2.44 ± 0.82 | 7.03 ± 1.3 | ||||||
| Mac-1-/- | 1.8 ± 0.16 | 2.4 ± 0.52 | 23.5 ± 1.1* | 3.89 ± 1.8 | 4.16 ± 2.2 | 0.95 ± 0.16 | 4.15 ± 0.82 | ||||||
. | Percentage of total cells ± SD . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 4 d after TG injection . | . | . | 6 d after TG injection . | . | 8 d after TG injection . | . | ||||||
| . | 0 h in vitro culture . | 20 h in vitro culture . | . | 0 h in vitro culture . | 20 h in vitro culture . | . | 20 h in vitro culture . | ||||||
. | . | –LPS/SB . | +LPS/SB . | . | . | 0 h in vitro culture . | . | ||||||
| WT | 2.3 ± 0.13 | 6.7 ± 0.56 | 46.9 ± 1.7 | 1.36 ± 1.1 | 1.43 ± 0.79 | 2.44 ± 0.82 | 7.03 ± 1.3 | ||||||
| Mac-1-/- | 1.8 ± 0.16 | 2.4 ± 0.52 | 23.5 ± 1.1* | 3.89 ± 1.8 | 4.16 ± 2.2 | 0.95 ± 0.16 | 4.15 ± 0.82 | ||||||
Macrophages were obtained from WT and Mac-1-/- mice at different time points after intraperitoneal injection of TG. The peritoneal macrophages were evaluated for apoptosis either immediately or after culturing in vitro for 20 hours in the presence or absence of LPS plus MAPK inhibitor SB202190. The extent of apoptosis was determined by dual-color FACS analysis using 7-AAD and annexin V. The apoptotic cells were defined as AAD-/annexin V+ and expressed as the percentage of total cells.
P < .05