Colony formation from BM cells of patients with JMML and healthy adults
. | No. of colonies formed, 2 × 104 BM NPMNCs . | . | GM colonies in total colonies, % . | . | ||
|---|---|---|---|---|---|---|
| Case no. . | GM-CSF absent . | GM-CSF present* . | GM-CSF absent . | GM-CSF present* . | ||
| Patients with JMML | ||||||
| 1 | 135 ± 23 | 160 ± 14 | 97 | 99 | ||
| 2 | 153 ± 33 | 190 ± 22 | 100 | 100 | ||
| 3 | 44 ± 12 | 121 ± 13 | 98 | 100 | ||
| 4 | 193 ± 34 | 168 ± 33 | 97 | 99 | ||
| 5 | 131 ± 32 | 177 ± 32 | 95 | 99 | ||
| 6 | 46 ± 11 | 94 ± 21 | 100 | 100 | ||
| 7 | 152 ± 21 | 206 ± 16 | 96 | 99 | ||
| 8 | 217 ± 31 | 198 ± 22 | 99 | 98 | ||
| Mean ± SD | 137 ± 41 | 160 ± 36 | 98 ± 2 | 99 ± 0.2 | ||
| Healthy adults | ||||||
| 1 | 0 ± 0 | 155 ± 22 | NA | 93 | ||
| 2 | 0 ± 0 | 28 ± 13 | NA | 65 | ||
| 3 | 0 ± 0 | 234 ± 35 | NA | 84 | ||
| 4 | 0 ± 0 | 104 ± 21 | NA | 91 | ||
| 5 | 0 ± 0 | 200 ± 22 | NA | 95 | ||
| 6 | 0 ± 0 | 203 ± 19 | NA | 97 | ||
| Mean ± SD | 0 ± 0 | 154 ± 23 | NA | 87 ± 11 | ||
. | No. of colonies formed, 2 × 104 BM NPMNCs . | . | GM colonies in total colonies, % . | . | ||
|---|---|---|---|---|---|---|
| Case no. . | GM-CSF absent . | GM-CSF present* . | GM-CSF absent . | GM-CSF present* . | ||
| Patients with JMML | ||||||
| 1 | 135 ± 23 | 160 ± 14 | 97 | 99 | ||
| 2 | 153 ± 33 | 190 ± 22 | 100 | 100 | ||
| 3 | 44 ± 12 | 121 ± 13 | 98 | 100 | ||
| 4 | 193 ± 34 | 168 ± 33 | 97 | 99 | ||
| 5 | 131 ± 32 | 177 ± 32 | 95 | 99 | ||
| 6 | 46 ± 11 | 94 ± 21 | 100 | 100 | ||
| 7 | 152 ± 21 | 206 ± 16 | 96 | 99 | ||
| 8 | 217 ± 31 | 198 ± 22 | 99 | 98 | ||
| Mean ± SD | 137 ± 41 | 160 ± 36 | 98 ± 2 | 99 ± 0.2 | ||
| Healthy adults | ||||||
| 1 | 0 ± 0 | 155 ± 22 | NA | 93 | ||
| 2 | 0 ± 0 | 28 ± 13 | NA | 65 | ||
| 3 | 0 ± 0 | 234 ± 35 | NA | 84 | ||
| 4 | 0 ± 0 | 104 ± 21 | NA | 91 | ||
| 5 | 0 ± 0 | 200 ± 22 | NA | 95 | ||
| 6 | 0 ± 0 | 203 ± 19 | NA | 97 | ||
| Mean ± SD | 0 ± 0 | 154 ± 23 | NA | 87 ± 11 | ||
NA indicates not applicable.
GM-CSF was used at a concentration of 10 ng/mL, which induced an optimal response among normal BM cells.