Genetic and functional analyses of B-cell lymphomas with 8p21.3 deletion
. | . | . | . | Methylation MSP . | . | . | . | Cell surface expression . | . | . | . | . | TRAIL-R1 polymorphisms . | . | . | . | TRAIL-R2 polymorphisms . | . | . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell line . | Origin . | Partial karyotype: chromosome 8 . | Deletion status . | TRAIL-R1 . | -R2 . | -R3 . | -R4 . | -R1 . | -R2 . | -R3 . | -R4 . | Sensitivity to TRAIL . | (A>G) 422 . | (G>C) 626 . | (A>C) 683 . | (G>A) 1322 . | (C>T) 200 . | (G>T) 485 . | (T>C) 572 . | |||||||||||
| JVM2 | PLL | der(8)t(3;8)(q11;p12) | del | U | U | M | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| MD-901 | DLBCL | der(8)t(8;13)(p11;q12), der(8)t(8;22)(q24.3;q11) | del | U | U | M | U | – | + | – | – | S | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| PR1 | FCL | der(8)t(8;16)(q24;q21), t(8;14;18)(q24;q32;q21),+der(6)t(6;7;8)(q21;?;?),+der(7)t(7;8)(q32;q24) | del | U | U | M | U | NA | NA | NA | NA | NA | G- | GC | AA | AA | C- | G- | C- | |||||||||||
| SUDHL6 | FCL | der(8)(8q24.1->8q10::8q10->8q24.1) | del | U | U | M | U | – | – | – | – | R | A- | G- | C- | A- | T- | G- | C- | |||||||||||
| 0Z | FCL | <3n>der(8)(22q13->22q11::8q24->8q11::8q10->8qter), der(22)t(8;22)(q24;q11) | del | U | U | U | U | – | – | – | – | R | A- | G- | C- | A- | C- | G- | C- | |||||||||||
| Oci-Ly8 | FCL | der(8)(17q25-17p12::8q13->8q24.3),der(8)t(8;14;18)(p23.2;q32;q21)t(3;8;14)(q 27;q24;q32), | del | U | U | M | U | NA | NA | NA | NA | NA | G- | C- | A- | A- | T- | G- | C- | |||||||||||
| NAMALWA | BL | t(8;14)(q24;q32) | del | U | NA | M | M | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| JVM13 | B-PLL | t(8;13)(p12;q13),+der(8)t(8;17)(p11;q11) | del | U | U | U | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| NCBE-1 | MCL | <4n>+del(8)(p11), +8 | del* | U | U | U | U | – | – | – | – | R | GG | CC | AA | AA | CC | GG | TT | |||||||||||
| REC-1 | MCL | der(8)t(2;8)(q31;p11), der(12)t(8;12)(q13;p12) | del | U | U | U | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| W133 | BL | t(8;14)(q24;q32), t(8;14;18)(q24;q32;q24) | del | U | U | M | U | NA | NA | NA | NA | NA | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| Z-138 | MCL | t(8;14)(q24;q32) | del | U | U | M | M | ++ | + | – | – | S | AG | GC | AA | AA | C- | G- | C- | |||||||||||
| G-519 | MCL | No chromosome alteration | no del | U | U | U | U | – | ++ | + | + | S | GG | CC | AA | AA | CT | GG | CC | |||||||||||
| HBL-2 | MCL | der(8)t(2;8)(q33;p23.3) | no del | U | U/M | M | U | ++ | ++ | – | + | S | GG | CC | AA | AA | CT | GG | CC | |||||||||||
| ELIJAH | BL | <4nt>(8;14)(q24;q32)×2 | no del | U/M | U | M | U | – | + | – | – | R | NA | NA | NA | NA | NA | NA | CC | |||||||||||
| VAL | FCL | t(8;14;18)(q24.3;q32.3;q21.3), +der(8)(18q23->18q21::14q32::8q24->8q10::8q10->8q24::14q32::18q21->18q23) | no del | U | U | M | U | + | ++ | – | + | S | AG | CC | AA | AA | CC | GG | CC | |||||||||||
| K422 | FCL | der(8)dup(8)(q22.3q24.3) | no del | NA | NA | NA | NA | ++ | ++ | – | + | S | GG | CC | AA | GG | CT | GG | CC | |||||||||||
| DOHH2 | FCL | t(8;14;18)(q24.3;q32.3;q21.3) | no del | U | NA | M | M | – | – | – | – | S | GG | CC | AA | GG | CT | GG | CC | |||||||||||
| BL-41 | BL | t(8;14)(q24;q32) | no del | NA | NA | NA | NA | + | – | – | – | S | NA | NA | NA | NA | NA | NA | NA | |||||||||||
| CA-46 | BL | t(8;14)(q24;q32) | no del | NA | NA | NA | NA | – | – | – | – | R | NA | NA | NA | NA | NA | NA | ||||||||||||
. | . | . | . | Methylation MSP . | . | . | . | Cell surface expression . | . | . | . | . | TRAIL-R1 polymorphisms . | . | . | . | TRAIL-R2 polymorphisms . | . | . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell line . | Origin . | Partial karyotype: chromosome 8 . | Deletion status . | TRAIL-R1 . | -R2 . | -R3 . | -R4 . | -R1 . | -R2 . | -R3 . | -R4 . | Sensitivity to TRAIL . | (A>G) 422 . | (G>C) 626 . | (A>C) 683 . | (G>A) 1322 . | (C>T) 200 . | (G>T) 485 . | (T>C) 572 . | |||||||||||
| JVM2 | PLL | der(8)t(3;8)(q11;p12) | del | U | U | M | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| MD-901 | DLBCL | der(8)t(8;13)(p11;q12), der(8)t(8;22)(q24.3;q11) | del | U | U | M | U | – | + | – | – | S | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| PR1 | FCL | der(8)t(8;16)(q24;q21), t(8;14;18)(q24;q32;q21),+der(6)t(6;7;8)(q21;?;?),+der(7)t(7;8)(q32;q24) | del | U | U | M | U | NA | NA | NA | NA | NA | G- | GC | AA | AA | C- | G- | C- | |||||||||||
| SUDHL6 | FCL | der(8)(8q24.1->8q10::8q10->8q24.1) | del | U | U | M | U | – | – | – | – | R | A- | G- | C- | A- | T- | G- | C- | |||||||||||
| 0Z | FCL | <3n>der(8)(22q13->22q11::8q24->8q11::8q10->8qter), der(22)t(8;22)(q24;q11) | del | U | U | U | U | – | – | – | – | R | A- | G- | C- | A- | C- | G- | C- | |||||||||||
| Oci-Ly8 | FCL | der(8)(17q25-17p12::8q13->8q24.3),der(8)t(8;14;18)(p23.2;q32;q21)t(3;8;14)(q 27;q24;q32), | del | U | U | M | U | NA | NA | NA | NA | NA | G- | C- | A- | A- | T- | G- | C- | |||||||||||
| NAMALWA | BL | t(8;14)(q24;q32) | del | U | NA | M | M | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| JVM13 | B-PLL | t(8;13)(p12;q13),+der(8)t(8;17)(p11;q11) | del | U | U | U | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| NCBE-1 | MCL | <4n>+del(8)(p11), +8 | del* | U | U | U | U | – | – | – | – | R | GG | CC | AA | AA | CC | GG | TT | |||||||||||
| REC-1 | MCL | der(8)t(2;8)(q31;p11), der(12)t(8;12)(q13;p12) | del | U | U | U | U | – | – | – | – | R | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| W133 | BL | t(8;14)(q24;q32), t(8;14;18)(q24;q32;q24) | del | U | U | M | U | NA | NA | NA | NA | NA | G- | C- | A- | A- | C- | G- | C- | |||||||||||
| Z-138 | MCL | t(8;14)(q24;q32) | del | U | U | M | M | ++ | + | – | – | S | AG | GC | AA | AA | C- | G- | C- | |||||||||||
| G-519 | MCL | No chromosome alteration | no del | U | U | U | U | – | ++ | + | + | S | GG | CC | AA | AA | CT | GG | CC | |||||||||||
| HBL-2 | MCL | der(8)t(2;8)(q33;p23.3) | no del | U | U/M | M | U | ++ | ++ | – | + | S | GG | CC | AA | AA | CT | GG | CC | |||||||||||
| ELIJAH | BL | <4nt>(8;14)(q24;q32)×2 | no del | U/M | U | M | U | – | + | – | – | R | NA | NA | NA | NA | NA | NA | CC | |||||||||||
| VAL | FCL | t(8;14;18)(q24.3;q32.3;q21.3), +der(8)(18q23->18q21::14q32::8q24->8q10::8q10->8q24::14q32::18q21->18q23) | no del | U | U | M | U | + | ++ | – | + | S | AG | CC | AA | AA | CC | GG | CC | |||||||||||
| K422 | FCL | der(8)dup(8)(q22.3q24.3) | no del | NA | NA | NA | NA | ++ | ++ | – | + | S | GG | CC | AA | GG | CT | GG | CC | |||||||||||
| DOHH2 | FCL | t(8;14;18)(q24.3;q32.3;q21.3) | no del | U | NA | M | M | – | – | – | – | S | GG | CC | AA | GG | CT | GG | CC | |||||||||||
| BL-41 | BL | t(8;14)(q24;q32) | no del | NA | NA | NA | NA | + | – | – | – | S | NA | NA | NA | NA | NA | NA | NA | |||||||||||
| CA-46 | BL | t(8;14)(q24;q32) | no del | NA | NA | NA | NA | – | – | – | – | R | NA | NA | NA | NA | NA | NA | ||||||||||||
Cell lines were derived from patients with B-cell prolymphocytic leukemia (B-PLL); mantle cell lymphoma (MCL); diffuse large B-cell lymphoma lacking t(14;18)(q32;q21) (DLBCL); diffuse large B-cell lymphoma with t(14;18)(q32;q21), follicle center lymphoma (FCL); and Burkitt lymphoma (BL). The genomic status of chromosome 8p was measured by array CGH and FISH. Cell surface expression of TRAIL receptors using flow cytometry. TRAIL-induced sensitivity: sensitive strain was defined when more than 25% of cells showed apoptosis after incubation with TRAIL ligand. For methylation-specific PCR, U indicates unmethylated promoter; M, methylated promoter; U/M, hemimethylated; NA, not analyzed.
The tetraploid NCBE-1 cell line showed deletion of only one 8p allele