Analysis of DC activation in terms of cytokine production and surface marker expression after incubation with supernatants of epithelial cells treated or not with the indicated bacteria
Bacterial strains . | IL-10, ng/mL . | IL-12p70, ng/mL . | CCL2, ng/mL . | CCL18, ng/mL . | CCL17, ng/mL . | CCL22, ng/mL . | HLADR+ cells, % . | DC-LAMPhi cells, % . | Invasive . | Flagellin . |
|---|---|---|---|---|---|---|---|---|---|---|
| SL 1344 WT | 2.46 | 0.3 | 5.1 | 61.8 | 0.36 | 12.0 | 95.1 | 90.6 | Yes | Yes |
| ssaV (SPI-II) | 2.99 | < DL | — | — | — | 13.0 | 92.8 | 96.0 | Yes | Yes |
| SL-FliC | 1.79 | 0.1 | < DL | 62.8 | 0.54 | 3.24 | 0.0 | — | Yes | No |
| msbB | 0.11 | < DL | 3.8 | — | — | — | — | 29.4 | No | Yes |
| SL-InvA (SPI-I) | 1.82 | 0.2 | 4.1 | 21.6 | 0.60 | 1.20 | 3.0 | 52.0 | No | Yes |
| DH5α | 0.392 | < DL | — | — | — | — | 2.0 | 33.4 | No | No |
| LP | 1.90 | 0.1 | < DL | 48.2 | 0.61 | 1.81 | 0.0 | — | No | No |
| Untreated EC supernatant | < DL | < DL | 1.4 | 36.2 | 0.24 | 1.14 | 4.0 | 28.0 | — | — |
| Untreated | < DL | < DL | 0.1 | 52.2 | 0.49 | 1.12 | 13.0 | 12.2 | — | — |
| DCs+SL 1344 WT | 2.263 | 1.3 | 3.2 | 48.0 | 0.54 | 27.29 | 95.0 | 98.8 | — | — |
Bacterial strains . | IL-10, ng/mL . | IL-12p70, ng/mL . | CCL2, ng/mL . | CCL18, ng/mL . | CCL17, ng/mL . | CCL22, ng/mL . | HLADR+ cells, % . | DC-LAMPhi cells, % . | Invasive . | Flagellin . |
|---|---|---|---|---|---|---|---|---|---|---|
| SL 1344 WT | 2.46 | 0.3 | 5.1 | 61.8 | 0.36 | 12.0 | 95.1 | 90.6 | Yes | Yes |
| ssaV (SPI-II) | 2.99 | < DL | — | — | — | 13.0 | 92.8 | 96.0 | Yes | Yes |
| SL-FliC | 1.79 | 0.1 | < DL | 62.8 | 0.54 | 3.24 | 0.0 | — | Yes | No |
| msbB | 0.11 | < DL | 3.8 | — | — | — | — | 29.4 | No | Yes |
| SL-InvA (SPI-I) | 1.82 | 0.2 | 4.1 | 21.6 | 0.60 | 1.20 | 3.0 | 52.0 | No | Yes |
| DH5α | 0.392 | < DL | — | — | — | — | 2.0 | 33.4 | No | No |
| LP | 1.90 | 0.1 | < DL | 48.2 | 0.61 | 1.81 | 0.0 | — | No | No |
| Untreated EC supernatant | < DL | < DL | 1.4 | 36.2 | 0.24 | 1.14 | 4.0 | 28.0 | — | — |
| Untreated | < DL | < DL | 0.1 | 52.2 | 0.49 | 1.12 | 13.0 | 12.2 | — | — |
| DCs+SL 1344 WT | 2.263 | 1.3 | 3.2 | 48.0 | 0.54 | 27.29 | 95.0 | 98.8 | — | — |
Bacterial strains: Invasive S typhimurium: wild type, SL 1344 WT; SPI-II (ssaV), FliC; noninvasive S typhimurium: msbB (lipid A mutant); SPI-I (InvA); E. coli: DH5a; L plantarum: LP.
< DL indicates below detection limit;—, not tested.