Comparison of 3 genomic amplification methods for the detection of parvovirus B19 DNA
. | . | No. positive*samples (%) . | . | . | ||
|---|---|---|---|---|---|---|
| Patient no. . | No. samples tested(%) . | VP1 PCR . | NS1 PCR . | QPCR . | ||
| 1 | 27 | 8 | 16 | 23 | ||
| 2 | 10 | 3 | 7 | 10 | ||
| 3 | 7 | 2 | 3 | 4 | ||
| 4 | 13 | 2 | 7 | 8 | ||
| 5 | 14 | 1 | 6 | 7 | ||
| 6 | 4 | 1 | 1 | 4 | ||
| Total | 75(100) | 17(23) | 40(53) | 56(75) | ||
. | . | No. positive*samples (%) . | . | . | ||
|---|---|---|---|---|---|---|
| Patient no. . | No. samples tested(%) . | VP1 PCR . | NS1 PCR . | QPCR . | ||
| 1 | 27 | 8 | 16 | 23 | ||
| 2 | 10 | 3 | 7 | 10 | ||
| 3 | 7 | 2 | 3 | 4 | ||
| 4 | 13 | 2 | 7 | 8 | ||
| 5 | 14 | 1 | 6 | 7 | ||
| 6 | 4 | 1 | 1 | 4 | ||
| Total | 75(100) | 17(23) | 40(53) | 56(75) | ||
A positive result was defined for PCR as at least 1 of 5 replicates positive and, for QPCR, 1 or 2 replicates positive.