The effect of Hx-heme on HO-1 and HO-2 mRNA transcription
Additive . | HO-1 . | HO-2 . |
|---|---|---|
| PBS | 1.2 ± 0.9 | 1.1 ± 0.9 |
| Heme + PBS | 174.5 ± 22.3 | 1.2 ± 0.3 |
| Hx-heme + PBS | 32.6 ± 9.5 | 0.9 ± 0.2 |
| Hx-heme + RAP | 17.4 ± 2.4 | 0.9 ± 0.3 |
| Hx-heme + anti-LRP/CD91 lgG | 11.6 ± 5.5 | 0.6 ± 0.4 |
| Hx-heme + nonimmune lgG | 25.9 ± 7.8 | 0.9 ± 0.1 |
Additive . | HO-1 . | HO-2 . |
|---|---|---|
| PBS | 1.2 ± 0.9 | 1.1 ± 0.9 |
| Heme + PBS | 174.5 ± 22.3 | 1.2 ± 0.3 |
| Hx-heme + PBS | 32.6 ± 9.5 | 0.9 ± 0.2 |
| Hx-heme + RAP | 17.4 ± 2.4 | 0.9 ± 0.3 |
| Hx-heme + anti-LRP/CD91 lgG | 11.6 ± 5.5 | 0.6 ± 0.4 |
| Hx-heme + nonimmune lgG | 25.9 ± 7.8 | 0.9 ± 0.1 |
The effect of Hx-heme on HO-1 and HO-2 mRNA levels in cultured monocytes as determined by reverse transcription-polymerase chain reaction. The cells were incubated for 6 hours at 37°C with 10 μM heme or with 10 μM Hx-heme in the absence or presence of 1 mg RAP, anti-LRP/CD91 lgG, and nonimmune lgG. The values shown are the mRNA levels relative to the levels before incubation. All values are the mean of triplicate determinations.