Summary of MDS/EVII retroviral vector insertions
Insertion no. . | Animal . | Vector* . | Date of transplantation . | LTR coordinate† . | Orientation‡ . | Sequence, LTR§ . | Lineages∥ . |
|---|---|---|---|---|---|---|---|
| 1 | RC5018 | LNL6/GINa | 5/15/98 | 170,623,356 | + | AATACACAGTAGTTTACCAGTAGTATACTCAAATTCAACTGGAGATGTACTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 2 | RC5018 | LNL6/GINa | 5/15/98 | 170,429,293 | + | CAATGGCACATTAATGCTCCCTCATGTCTCTGCCCAGAAGGTGTTCAACATGAAAGACCC | Identified in Grans; T cells +, B cells + |
| 3 | 96E0257 | LNL6/GINa | 7/30/99 | 170,213,060 | − | GGCAATGATCCTACCACCAACCAGTTCCTTCTGGCACTGTTGATACTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 4 | RC7067 | LNL6/GINa | 1/14/00 | 170,273,870 | + | AGAGCACATTAGACTGAGTGGTTTATTTACAAGTAAAAATCACATTGATGTGAAAGACCC | Identified in DCs; Gran +, T cells −, B cells − |
| 5 | RQ22977 | LNL6/GINa | 7/7/00 | 170,362,372 | − | GACCACAGTCTTTCTGCCCAGGAGGCCACCAAAGGGCCTGAATGTTGCCTTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 6 | RQ22977 | LNL6/GINa | 7/7/00 | 170,351,399 | + | TCCCTAGACCTGCTCCTCCTATTGTCCTCGAATATTCTATTAGGTGCTGATGAAAGACCC | Identified in Grans |
| 7 | RQ23265 | GINa, MPSV-G6PD | 8/12/00 | 170,362,387 | − | GCCCAGGAGGCCACCAAAGGGTCTGAATGTTGCCACTACAGTCCTACCCTTGAAAGACCC | Identified in Grans |
| 8 | RQ23265 | GINa, MPSV-G6PD | 8/12/00 | 170,361,790 | + | TGCATGTCTGAGGTCTAACAGATTGGTCCCAGGATATTGCATGTTTTCCCTGAAAGACCC | Identified in Grans |
| 9 | RQ22776 | LNL6/GINa | 2/9/01 | 170,278,164 | + | TAGGTTTCCCTTGATGTGAACTTTTCAAGCTATTTCTTTTTGATAGCTATTGAAAGACCC | Identified in Grans |
| 10 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,349,678 | + | TTCCCACGGCTTTCGTGTTTGGATATTACTGTATCTTCTCAATCAGATGTTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 11 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,273,453 | + | AGTGGCAAAGAAAGCATAAGTATTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 12 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,373,081 | − | AATTAACAGATATGCAAAACAAGGTAAAGCACCGGACATTATTAGCTCATTCTGAAAGACCC | Identified in Grans; T cells +, B cells − |
| 13 | RQ24288 | LNL6/GINa | 3/15/02 | 170,699,486 | + | TGGGTAATAAAGGAAATCATACTAAAACTACAGATTTTTGTATTTTAAAGTGAAAGACCC | Identified in Grans |
| 14 | RC9036 | L6/GINa | 7/10/02 | 170,393,825 | − | TGTCGGGAAAAAAGGAAGCCACTTAATGGCTCACAATAGATCTTGAAAGACCC | Identified in T cells |
Insertion no. . | Animal . | Vector* . | Date of transplantation . | LTR coordinate† . | Orientation‡ . | Sequence, LTR§ . | Lineages∥ . |
|---|---|---|---|---|---|---|---|
| 1 | RC5018 | LNL6/GINa | 5/15/98 | 170,623,356 | + | AATACACAGTAGTTTACCAGTAGTATACTCAAATTCAACTGGAGATGTACTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 2 | RC5018 | LNL6/GINa | 5/15/98 | 170,429,293 | + | CAATGGCACATTAATGCTCCCTCATGTCTCTGCCCAGAAGGTGTTCAACATGAAAGACCC | Identified in Grans; T cells +, B cells + |
| 3 | 96E0257 | LNL6/GINa | 7/30/99 | 170,213,060 | − | GGCAATGATCCTACCACCAACCAGTTCCTTCTGGCACTGTTGATACTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 4 | RC7067 | LNL6/GINa | 1/14/00 | 170,273,870 | + | AGAGCACATTAGACTGAGTGGTTTATTTACAAGTAAAAATCACATTGATGTGAAAGACCC | Identified in DCs; Gran +, T cells −, B cells − |
| 5 | RQ22977 | LNL6/GINa | 7/7/00 | 170,362,372 | − | GACCACAGTCTTTCTGCCCAGGAGGCCACCAAAGGGCCTGAATGTTGCCTTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 6 | RQ22977 | LNL6/GINa | 7/7/00 | 170,351,399 | + | TCCCTAGACCTGCTCCTCCTATTGTCCTCGAATATTCTATTAGGTGCTGATGAAAGACCC | Identified in Grans |
| 7 | RQ23265 | GINa, MPSV-G6PD | 8/12/00 | 170,362,387 | − | GCCCAGGAGGCCACCAAAGGGTCTGAATGTTGCCACTACAGTCCTACCCTTGAAAGACCC | Identified in Grans |
| 8 | RQ23265 | GINa, MPSV-G6PD | 8/12/00 | 170,361,790 | + | TGCATGTCTGAGGTCTAACAGATTGGTCCCAGGATATTGCATGTTTTCCCTGAAAGACCC | Identified in Grans |
| 9 | RQ22776 | LNL6/GINa | 2/9/01 | 170,278,164 | + | TAGGTTTCCCTTGATGTGAACTTTTCAAGCTATTTCTTTTTGATAGCTATTGAAAGACCC | Identified in Grans |
| 10 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,349,678 | + | TTCCCACGGCTTTCGTGTTTGGATATTACTGTATCTTCTCAATCAGATGTTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 11 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,273,453 | + | AGTGGCAAAGAAAGCATAAGTATTGAAAGACCC | Identified in Grans; T cells −, B cells − |
| 12 | RQ22879 | LNL6/GINa, GIPL1 | 3/30/01 | 170,373,081 | − | AATTAACAGATATGCAAAACAAGGTAAAGCACCGGACATTATTAGCTCATTCTGAAAGACCC | Identified in Grans; T cells +, B cells − |
| 13 | RQ24288 | LNL6/GINa | 3/15/02 | 170,699,486 | + | TGGGTAATAAAGGAAATCATACTAAAACTACAGATTTTTGTATTTTAAAGTGAAAGACCC | Identified in Grans |
| 14 | RC9036 | L6/GINa | 7/10/02 | 170,393,825 | − | TGTCGGGAAAAAAGGAAGCCACTTAATGGCTCACAATAGATCTTGAAAGACCC | Identified in T cells |
−indicates below limit of detection or more than 2 logs lower than granulocyte lineage signal; + indicates present.
Vector or vectors used to transduce autologous CD34+ cells.
Site of vector insertion, based on the humen July 2003 assembly.
Orientation of the inserted provirus in relation to the Mds1/Evi1 genes: + indicates provirus in same orientation as the genes; − indicates provirus in opposite orientation as the genes.
Junction of genomic DNA (roman typeface) and the proviral 5′ LTR (bold italic typeface).
Lineage in which Mds1/Evi1 insertion initially identified via LAM-PCR and sequencing given in first line, lineage analysis via allele-specific primers given on second line.