IDO expression and activity
. | IDO mRNA (qPCR)* . | . | . | . | IDO activity (μM kynurenine, HPLC) . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Conditions . | n . | Average . | Range . | SEM . | n . | Average . | Range . | SEM . | ||||||
| iDC | 7 | 0.017 | 0.09-0.06 | 0.007 | 6 | 4.3 | 2.8-7.7 | 0.8 | ||||||
| TNFα + PGE2 | 7 | 3.3 | 1.01-8.17 | 1.01 | 6 | 38 | 19.3-68.9 | 7.6 | ||||||
| LPS | 4 | 0.24 | 0.09-0.39 | 0.06 | 3 | 7.6 | 2.8-14 | 3.3 | ||||||
| SAC | 3 | 0.05 | 0-0.13 | 0.04 | 1 | 4.3 | N/A | N/A | ||||||
| TNFα + PGE2 + IFN-γ | 1 | 5.1 | N/A | N/A | 3 | 66.5 | 60.4-74 | 4.0 | ||||||
. | IDO mRNA (qPCR)* . | . | . | . | IDO activity (μM kynurenine, HPLC) . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Conditions . | n . | Average . | Range . | SEM . | n . | Average . | Range . | SEM . | ||||||
| iDC | 7 | 0.017 | 0.09-0.06 | 0.007 | 6 | 4.3 | 2.8-7.7 | 0.8 | ||||||
| TNFα + PGE2 | 7 | 3.3 | 1.01-8.17 | 1.01 | 6 | 38 | 19.3-68.9 | 7.6 | ||||||
| LPS | 4 | 0.24 | 0.09-0.39 | 0.06 | 3 | 7.6 | 2.8-14 | 3.3 | ||||||
| SAC | 3 | 0.05 | 0-0.13 | 0.04 | 1 | 4.3 | N/A | N/A | ||||||
| TNFα + PGE2 + IFN-γ | 1 | 5.1 | N/A | N/A | 3 | 66.5 | 60.4-74 | 4.0 | ||||||
N/A indicates not applicable.
Reported as a ratio of relative mRNA expression IDO/GAPDH. Non-normalized data are presented