Table 1. Fludarabine-induced apoptosis... | American Society of Hematology

Table 1.

Fludarabine-induced apoptosis in CD4⁺ CD25⁺ and CD4⁺ CD25^– T cells

	Experiment 1			Experiment 2
	Medium	Medium plus fludarabine	Medium	Medium plus fludarabine
CD4⁺ CD25^–, %	91	66	90	52
CD4⁺ CD25⁺, %	69	23	79	30

	Experiment 1			Experiment 2
	Medium	Medium plus fludarabine	Medium	Medium plus fludarabine
CD4⁺ CD25^–, %	91	66	90	52
CD4⁺ CD25⁺, %	69	23	79	30

CD4⁺ CD25⁺ T cells are more susceptible to fludarabine-induced apoptosis than CD4⁺ CD25^– T cells. PBMCs from 2 different donors were incubated in vitro with medium alone or with medium containing 10 μM fludarabine. After 48 hours cells were stained with 7-AAD and annexin V and viable cells were defined as 7-AAD and annexin-V double-negative cells. Viable cells are listed as percentage of the respective cell type.

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