PCR primers and probes
Genes . | Sequences . | Sizes, bp . |
|---|---|---|
| SARS-CoV | (F) 5′ TAC ACA CCT CAG CGT TG 3′ | 182 |
| (R) 5′ CAC GAA CGT GAC GAA T 3′ | ||
| β-actin | (F) 5′ CCC AAG GCC AAC CGC GAG AAG AT 3′ | 219 |
| (R) 5′ GTC CCG GCC AGC CAG GTC CAG 3′ | ||
| IFN-α | (F) 5′ CCT TCC TCC TGT CTG ATG GA 3′ | 67 |
| (P) 5′ (FAM) CAG ACA TGA CTT TGG ATT TCC CCA GG (TARMA) 3′ | ||
| (R) 5′ ACT GGT TGC CAT CAA ACT CC 3′ | ||
| IFN-β | (F) 5′ GCC GCA TTG ACC ATC T 3′ | 261 |
| (R) 5′ AGG AGT ACA GTC ACT GTG 3′ | ||
| IFN-γ | (F) 5′ CTA ATT ATT CGG TAA CTG ACT TGA 3′ | 75 |
| (P) 5′ (FAM) TCC AAC GCA AAG CAA TAC ATG AAC (TARMA) 3′ | ||
| (R) 5′ ACA GTT CAG CCA TCA CTT GGA 3′ | ||
| TNF-α | (F) 5′ GGC TCC AGG CGG TGC TTG TTC 3′ | 409 |
| (R) 5′ AGA CGG CGA TGC GGC TGA TG 3′ | ||
| IL-6 | (F) 5′ ATT CGG TAC ATC CTC GAC 3′ | 331 |
| (R) 5′ GGG GTG GTT ATT GCA TC 3′ | ||
| IL-12 | (F) 5′ CGG TCA TCT GCC GCA AA 3′ | 80 |
| (R) 5′ TGC CCA TTC GCT CCA AGA 3′ | ||
| MIP-1α | (F) 5′ CTC TGC ACC ATG GCT CTC TGC AAC 3′ | 98 |
| (R) 5′ TGT GGA ATC TGC CGG GAG GTG TAG 3′ | ||
| RANTES | (F) 5′ CCC CTC ACT ATC CTA CC 3′ | 285 |
| (R) 5′ TCA CGC CAT TCT CCT G 3′ | ||
| IP-10 | (F) 5′ CTG ACT CTA AGT GGC ATT 3′ | 208 |
| (R) 5′ TGA TGG CCT TCG ATT CTG 3′ | ||
| MCP-1 | (F) 5′ CAT TGT GGC CAA GGA GAT CTG 3′ | 91 |
| (R) 5′ CTT CGG AGT TTG GGT TTG CTT 3′ | ||
| ACE2 | (F) 5′ CTG GGA TCA GAG ATC GGA AGA 3′ | 288 |
| (R) 5′ CTC TCT CCT TGG CCA TGT TGT 3′ |
Genes . | Sequences . | Sizes, bp . |
|---|---|---|
| SARS-CoV | (F) 5′ TAC ACA CCT CAG CGT TG 3′ | 182 |
| (R) 5′ CAC GAA CGT GAC GAA T 3′ | ||
| β-actin | (F) 5′ CCC AAG GCC AAC CGC GAG AAG AT 3′ | 219 |
| (R) 5′ GTC CCG GCC AGC CAG GTC CAG 3′ | ||
| IFN-α | (F) 5′ CCT TCC TCC TGT CTG ATG GA 3′ | 67 |
| (P) 5′ (FAM) CAG ACA TGA CTT TGG ATT TCC CCA GG (TARMA) 3′ | ||
| (R) 5′ ACT GGT TGC CAT CAA ACT CC 3′ | ||
| IFN-β | (F) 5′ GCC GCA TTG ACC ATC T 3′ | 261 |
| (R) 5′ AGG AGT ACA GTC ACT GTG 3′ | ||
| IFN-γ | (F) 5′ CTA ATT ATT CGG TAA CTG ACT TGA 3′ | 75 |
| (P) 5′ (FAM) TCC AAC GCA AAG CAA TAC ATG AAC (TARMA) 3′ | ||
| (R) 5′ ACA GTT CAG CCA TCA CTT GGA 3′ | ||
| TNF-α | (F) 5′ GGC TCC AGG CGG TGC TTG TTC 3′ | 409 |
| (R) 5′ AGA CGG CGA TGC GGC TGA TG 3′ | ||
| IL-6 | (F) 5′ ATT CGG TAC ATC CTC GAC 3′ | 331 |
| (R) 5′ GGG GTG GTT ATT GCA TC 3′ | ||
| IL-12 | (F) 5′ CGG TCA TCT GCC GCA AA 3′ | 80 |
| (R) 5′ TGC CCA TTC GCT CCA AGA 3′ | ||
| MIP-1α | (F) 5′ CTC TGC ACC ATG GCT CTC TGC AAC 3′ | 98 |
| (R) 5′ TGT GGA ATC TGC CGG GAG GTG TAG 3′ | ||
| RANTES | (F) 5′ CCC CTC ACT ATC CTA CC 3′ | 285 |
| (R) 5′ TCA CGC CAT TCT CCT G 3′ | ||
| IP-10 | (F) 5′ CTG ACT CTA AGT GGC ATT 3′ | 208 |
| (R) 5′ TGA TGG CCT TCG ATT CTG 3′ | ||
| MCP-1 | (F) 5′ CAT TGT GGC CAA GGA GAT CTG 3′ | 91 |
| (R) 5′ CTT CGG AGT TTG GGT TTG CTT 3′ | ||
| ACE2 | (F) 5′ CTG GGA TCA GAG ATC GGA AGA 3′ | 288 |
| (R) 5′ CTC TCT CCT TGG CCA TGT TGT 3′ |
(F) indicates forward primers; (R), reverse primers; (P), Taqman probes; TNF, tumor necrosis factor; IFN, interferon; IL, interleukin; MIP, macrophage inflammatory protein; RANTES, regulated on activation, normal T cell expressed and secreted; IP-10, interferon-γ induced protein 10; MCP-1, monocyte chemoattractant protein 1, ACE2, angiotensin converting enzyme 2.