Number of cells in each fraction of the B lineage
Parameter* . | PU.1+/+CD19 . | PU.1Δ/ΔCD19 . |
|---|---|---|
| Bone marrow | ||
| Total cells | 20.4 × 106 ± 1.7 × 106 | 17.7 × 106 ± 4.2 × 106 |
| Pre-BI, % | 1.9 ± 0.4 | 1.4 ± 0.3 |
| Pre-BII, % | 16.5 ± 4.7 | 23.1 ± 5.7 |
| Immature B, % | 6.5 ± 0.9 | 9.3 ± 2.3 |
| Mature recirculating, % | 6.5 ± 2.1 | 2.6 ± 0.4 |
| Spleen | ||
| Total cells | 54.7 × 106 ± 6.9 × 106 | 49.4 × 106 ± 5.3 × 106 |
| Transitional 1, % | 1.8 ± 0.5 | 4.8 ± 1.7 |
| Transitional 2, % | 1.8 ± 0.5 | 3.9 ± 1.1 |
| Follicular, % | 33.7 ± 2.5 | 17 ± 3.4 |
| Marginal zone, % | 1.1 ± 0.4 | 1.7 ± 0.5 |
| Lymph nodes | ||
| Total cells | 6.5 × 106 ± 7.6 × 105 | 5.8 × 106 ± 5.4 × 105 |
| B cells, % | 52.2 ± 4.6 | 28.5 ± 5.9 |
| Peritoneal cells | ||
| Total cells | 7.3 × 106 ± 1.6 × 106 | 5.8 × 106 ± 1.4 × 106 |
| B1 cells, % | 28.5 ± 3.3 | 36.8 ± 7.6 |
Parameter* . | PU.1+/+CD19 . | PU.1Δ/ΔCD19 . |
|---|---|---|
| Bone marrow | ||
| Total cells | 20.4 × 106 ± 1.7 × 106 | 17.7 × 106 ± 4.2 × 106 |
| Pre-BI, % | 1.9 ± 0.4 | 1.4 ± 0.3 |
| Pre-BII, % | 16.5 ± 4.7 | 23.1 ± 5.7 |
| Immature B, % | 6.5 ± 0.9 | 9.3 ± 2.3 |
| Mature recirculating, % | 6.5 ± 2.1 | 2.6 ± 0.4 |
| Spleen | ||
| Total cells | 54.7 × 106 ± 6.9 × 106 | 49.4 × 106 ± 5.3 × 106 |
| Transitional 1, % | 1.8 ± 0.5 | 4.8 ± 1.7 |
| Transitional 2, % | 1.8 ± 0.5 | 3.9 ± 1.1 |
| Follicular, % | 33.7 ± 2.5 | 17 ± 3.4 |
| Marginal zone, % | 1.1 ± 0.4 | 1.7 ± 0.5 |
| Lymph nodes | ||
| Total cells | 6.5 × 106 ± 7.6 × 105 | 5.8 × 106 ± 5.4 × 105 |
| B cells, % | 52.2 ± 4.6 | 28.5 ± 5.9 |
| Peritoneal cells | ||
| Total cells | 7.3 × 106 ± 1.6 × 106 | 5.8 × 106 ± 1.4 × 106 |
| B1 cells, % | 28.5 ± 3.3 | 36.8 ± 7.6 |
The percentage of the total cell number in the bone marrow (femur and tibia of both hind legs), spleen, lymph nodes (axial and inguinal), and peritoneum (peritoneal lavage) for each fraction within the B lineage was assessed by flow cytometry. The antibody combinations used were as follows: bone marrow: pre-BI (B220+c-Kit+), pre-BII (B220+c-Kit−CD25+), immature B (B220loIgM+), and mature recirculating (B220hiIgM+); spleen: transitional 1 (CD23−IgM+CD21lo), transitional 2 (CD23+IgMhiCD21hi), follicular (CD23+IgMloCD21lo), and marginal zone (CD23−IgM+CD21hi). B cells (CD19+IgM+) from the lymph nodes and B1 cells (Mac-1+B220?) in the peritoneum.
For PU.1+/+CD19; n = 5; for PU.1Δ/ΔCD19; n = 5.