Detection of antineutrophil antibodies.
| Granulocyte immunofluorescence test (GIFT) | Detection of neutrophil-bound antibody by binding of glutaraldehyde- fixed patient neutrophils to fluorescently labeled anti-human IgG |
| Granulocyte indirect immunofluorescence test (GIIFT) | Detection of serum antibody by incubation with normal neutrophils, or specifically phenotyped neutrophils, with subsequent staining with fluorescently labeled anti-human IgG |
| Granulocyte agglutination test (GAT) | Incubation of granulocytes with patient sera and microscopic evaluation of agglutination |
| Enzyme linked immunoassays (ELISA) | Detection of antibody in serum by binding to glutaraldehyde-fixed normal neutrophils on microtiter plates with detection by conjugated anti-human IgG |
| Monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) | Simultaneous incubation of defined neutrophils with patient serum and monoclonal antibodies directed against neutrophil-specific antigens. Antigens are then immobilized on column coated with anti-mouse ab, and assayed for presence of human antibody |
| Granulocyte immunofluorescence test (GIFT) | Detection of neutrophil-bound antibody by binding of glutaraldehyde- fixed patient neutrophils to fluorescently labeled anti-human IgG |
| Granulocyte indirect immunofluorescence test (GIIFT) | Detection of serum antibody by incubation with normal neutrophils, or specifically phenotyped neutrophils, with subsequent staining with fluorescently labeled anti-human IgG |
| Granulocyte agglutination test (GAT) | Incubation of granulocytes with patient sera and microscopic evaluation of agglutination |
| Enzyme linked immunoassays (ELISA) | Detection of antibody in serum by binding to glutaraldehyde-fixed normal neutrophils on microtiter plates with detection by conjugated anti-human IgG |
| Monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) | Simultaneous incubation of defined neutrophils with patient serum and monoclonal antibodies directed against neutrophil-specific antigens. Antigens are then immobilized on column coated with anti-mouse ab, and assayed for presence of human antibody |