HMCL characteristics and response to MV-GFP
| HMCL characteristics . | MV-GFP response . | |||||
|---|---|---|---|---|---|---|
| p53 status and name . | TP53 . | RAS (H/N/K) . | CD46 MFIR . | GFP, % . | Cell death, % . | IFN-α, pg/mL . |
| TP53wt | ||||||
| NAN9 | WT | WT | 21 | 4.5 | 0.3 | 0 |
| SBN | WT | WT | 6 | 0.1 | 1.6 | 0 |
| BCN | WT | WT | 64 | 36.5 | 63.6 | 550 |
| AMO1 | WT | K_A146T | 11 | 1.1 | 1.0 | 0 |
| MDN | WT | N_G13D | 28 | 4.4 | 0.6 | 0 |
| MM1S | WT | K_G12A | 110 | 22.9 | 29.0 | 8 |
| NAN11 | WT | H_G13V | 76 | 15.5 | 21.0 | 80 |
| NCI-H929 | WT | N_G13D | 27 | 1.6 | 4.2 | 0 |
| XG3 | WT | N_Q61K | 17 | 1.6 | 0.5 | 0 |
| XG6 | WT | K_Q61E | 80 | 0.7 | 1.8 | 0 |
| XG7 | WT | K_G12C | 33 | 61.5 | 28.2 | 350 |
| XG10 | WT | K_G13C | 20 | 17.0 | 57.0 | 0 |
| TP53abn | ||||||
| ANBL6 | Q331*/− | WT | 31 | 8.5 | 11.0 | 0 |
| JIM3 | R273C/− | WT | 30 | 61.3 | 20.9 | 0 |
| KMS11 | −/− | WT | 137 | 72.7 | 84.5 | 0 |
| KMS12BM | R337L/− | WT | 53 | 79.1 | 14.1 | 80 |
| KMS12PE | R337L/− | WT | 45 | 60.0 | 31.7 | 34 |
| LP1 | E286K/− | WT | 48 | 81.6 | 44.0 | 160 |
| NAN6 | Δ exons 7-8-9/− | WT | 55 | 11.0 | 10.0 | 0 |
| NAN8 | D21Y/- | WT | 22 | 1.0 | 6.2 | 0 |
| OPM2 | R175H/− | WT | 38 | 11.6 | 7.0 | 0 |
| SKMM2 | K132N/− | WT | 30 | 20.5 | 13.5 | 17 |
| XG5 | R282W/− | WT | 105 | 39.5 | 21.3 | 0 |
| NAN1 | E180*/− | WT | 21 | 10.0 | 13.5 | 0 |
| U266 | A161T/− | WT | 17 | 5.6 | 19.1 | 43 |
| JJN3 | −/− | N_G12D | 66 | 47.7 | 49.3 | 0 |
| KARPAS620 | C135Y/− | K_G12D | 56 | 9.3 | 8.9 | 450 |
| KMM1 | C135F/S241F | N_G12A | 62 | 17.4 | 20.9 | 0 |
| L363 | S261T/− | N_Q61H | 54 | 14.4 | 12.3 | 0 |
| NAN10 | A161D/− | N_G12R | 51 | 13.8 | 4.6 | 0 |
| NAN3 | R248Q/− | N_Q61K | 80 | 5.0 | 17.0 | 0 |
| NAN7 | Δ exon 11/− | N_G12D | 29 | 1.0 | 1.6 | 0 |
| RPMI8226 | E285K/− | K_G12A | 165 | 48.5 | 31.0 | 0 |
| XG1 | Y126N/− | N_G12R | 100 | 28.5 | 14.4 | 96 |
| XG2 | C176Y/R213* | K_G12A | 145 | 5.5 | 13.0 | 0 |
| XG11 | C135Y/− | K_K117N | 83 | 66.7 | 65.9 | 0 |
| XG16 | Y220C/− | N_Q61H | 87 | 15.0 | 22.0 | 0 |
| HMCL characteristics . | MV-GFP response . | |||||
|---|---|---|---|---|---|---|
| p53 status and name . | TP53 . | RAS (H/N/K) . | CD46 MFIR . | GFP, % . | Cell death, % . | IFN-α, pg/mL . |
| TP53wt | ||||||
| NAN9 | WT | WT | 21 | 4.5 | 0.3 | 0 |
| SBN | WT | WT | 6 | 0.1 | 1.6 | 0 |
| BCN | WT | WT | 64 | 36.5 | 63.6 | 550 |
| AMO1 | WT | K_A146T | 11 | 1.1 | 1.0 | 0 |
| MDN | WT | N_G13D | 28 | 4.4 | 0.6 | 0 |
| MM1S | WT | K_G12A | 110 | 22.9 | 29.0 | 8 |
| NAN11 | WT | H_G13V | 76 | 15.5 | 21.0 | 80 |
| NCI-H929 | WT | N_G13D | 27 | 1.6 | 4.2 | 0 |
| XG3 | WT | N_Q61K | 17 | 1.6 | 0.5 | 0 |
| XG6 | WT | K_Q61E | 80 | 0.7 | 1.8 | 0 |
| XG7 | WT | K_G12C | 33 | 61.5 | 28.2 | 350 |
| XG10 | WT | K_G13C | 20 | 17.0 | 57.0 | 0 |
| TP53abn | ||||||
| ANBL6 | Q331*/− | WT | 31 | 8.5 | 11.0 | 0 |
| JIM3 | R273C/− | WT | 30 | 61.3 | 20.9 | 0 |
| KMS11 | −/− | WT | 137 | 72.7 | 84.5 | 0 |
| KMS12BM | R337L/− | WT | 53 | 79.1 | 14.1 | 80 |
| KMS12PE | R337L/− | WT | 45 | 60.0 | 31.7 | 34 |
| LP1 | E286K/− | WT | 48 | 81.6 | 44.0 | 160 |
| NAN6 | Δ exons 7-8-9/− | WT | 55 | 11.0 | 10.0 | 0 |
| NAN8 | D21Y/- | WT | 22 | 1.0 | 6.2 | 0 |
| OPM2 | R175H/− | WT | 38 | 11.6 | 7.0 | 0 |
| SKMM2 | K132N/− | WT | 30 | 20.5 | 13.5 | 17 |
| XG5 | R282W/− | WT | 105 | 39.5 | 21.3 | 0 |
| NAN1 | E180*/− | WT | 21 | 10.0 | 13.5 | 0 |
| U266 | A161T/− | WT | 17 | 5.6 | 19.1 | 43 |
| JJN3 | −/− | N_G12D | 66 | 47.7 | 49.3 | 0 |
| KARPAS620 | C135Y/− | K_G12D | 56 | 9.3 | 8.9 | 450 |
| KMM1 | C135F/S241F | N_G12A | 62 | 17.4 | 20.9 | 0 |
| L363 | S261T/− | N_Q61H | 54 | 14.4 | 12.3 | 0 |
| NAN10 | A161D/− | N_G12R | 51 | 13.8 | 4.6 | 0 |
| NAN3 | R248Q/− | N_Q61K | 80 | 5.0 | 17.0 | 0 |
| NAN7 | Δ exon 11/− | N_G12D | 29 | 1.0 | 1.6 | 0 |
| RPMI8226 | E285K/− | K_G12A | 165 | 48.5 | 31.0 | 0 |
| XG1 | Y126N/− | N_G12R | 100 | 28.5 | 14.4 | 96 |
| XG2 | C176Y/R213* | K_G12A | 145 | 5.5 | 13.0 | 0 |
| XG11 | C135Y/− | K_K117N | 83 | 66.7 | 65.9 | 0 |
| XG16 | Y220C/− | N_Q61H | 87 | 15.0 | 22.0 | 0 |
The response to MV-GFP response was assessed 4 days after infection (MOI = 1) using flow cytometry. Infected cells were determined using GFP fluorescence (expressed as the percentage of positive cells). Cell death was measured using Live/Dead staining or by the altered morphology (reduced FSC and increased SSC). Results represent the mean of 4 to 5 independent experiments. CD46 expression was expressed as the mean fluorescence intensity ratio (MFIR). CD46 and control MFI values are provided in supplemental Table 1A).
−, deletion; WT, wild-type.