Oligonucleotide primers used for site-directed mutagenesis
| Mutation . | Orientation . | Primer sequence . |
|---|---|---|
| Mouse GPIIIa | ||
| T30A S32PQ33L | Forward | 5′-gtgctcagatgagGcCttgCctcTgggctcaccccgatg-3′ |
| Reverse | 5′-catcggggtgagcccAgagGcaaGgCctcatctgagcac-3′ | |
| N39D | Forward | 5′-gggctcaccccgatgtGacctgaaggagaacctg-3′ |
| Reverse | 5′-caggttctccttcaggtCacatcggggtgagccc-3′ | |
| M470Q | Forward | 5′-gaccagggctggctggggtccCAgtgtgagtgctctgaggagg-3′ |
| Reverse | 5′-cctcctcagagcactcacacTGggaccccagccagccctggtc-3′ | |
| P446H | Forward | 5′-gaccccagccagccagggccACagcggcacac-3′ |
| Reverse | 5′-ggtgtgccgctGTggccctggctggctggggtcc-3′ | |
| Human GPIIIa | ||
| Q470M | Forward | 5′-gggcctggctggctgggatccATGtgtgagtgctcagaggaggac-3′ |
| Reverse | 5′-gtcctcctctgagcactcacaCATggatcccagccagccaggccc-3′ | |
| H446P | Forward | 5′-ctgaacctaatagccCtcgctgcaacaatgg-3′ |
| Reverse | 5′-ccattgttgcagcgaGggctattaggttcag-3′ | |
| G463D P464Q | Forward | 5′-gtggggtatgccgttgtgACcAGggctggctgggatcccag-3′ |
| Reverse | 5′-ctgggatcccagccagccCTgGTcacaacggcataccccac-3′ |
| Mutation . | Orientation . | Primer sequence . |
|---|---|---|
| Mouse GPIIIa | ||
| T30A S32PQ33L | Forward | 5′-gtgctcagatgagGcCttgCctcTgggctcaccccgatg-3′ |
| Reverse | 5′-catcggggtgagcccAgagGcaaGgCctcatctgagcac-3′ | |
| N39D | Forward | 5′-gggctcaccccgatgtGacctgaaggagaacctg-3′ |
| Reverse | 5′-caggttctccttcaggtCacatcggggtgagccc-3′ | |
| M470Q | Forward | 5′-gaccagggctggctggggtccCAgtgtgagtgctctgaggagg-3′ |
| Reverse | 5′-cctcctcagagcactcacacTGggaccccagccagccctggtc-3′ | |
| P446H | Forward | 5′-gaccccagccagccagggccACagcggcacac-3′ |
| Reverse | 5′-ggtgtgccgctGTggccctggctggctggggtcc-3′ | |
| Human GPIIIa | ||
| Q470M | Forward | 5′-gggcctggctggctgggatccATGtgtgagtgctcagaggaggac-3′ |
| Reverse | 5′-gtcctcctctgagcactcacaCATggatcccagccagccaggccc-3′ | |
| H446P | Forward | 5′-ctgaacctaatagccCtcgctgcaacaatgg-3′ |
| Reverse | 5′-ccattgttgcagcgaGggctattaggttcag-3′ | |
| G463D P464Q | Forward | 5′-gtggggtatgccgttgtgACcAGggctggctgggatcccag-3′ |
| Reverse | 5′-ctgggatcccagccagccCTgGTcacaacggcataccccac-3′ |
Altered sequences are in upper case.