Site of α-granule fusion pore anchoring following thrombin stimulation
| . | Thrombin stimulation . | |
|---|---|---|
| 90 s, 0.1 U/mL . | 300 s, 0.1 U/mL . | |
| PM anchored | ||
| Single decondensed α-granule | 31% | 35% |
| N = 21 | N = 32 | |
| Compound fused decondensed α-granule string | 30% | 29% |
| N = 20 | N = 26 | |
| CS anchored | ||
| Single decondensed α-granule anchored to CS pore | 15% | 11% |
| N = 10 | N = 10 | |
| Unanchored | ||
| Single decondensed α-granule | 1% | 12% |
| N = 1 | N = 11 | |
| Compound fused decondensed α-granule string | 22% | 13% |
| N = 14 | N = 12 | |
| Relative frequency | Total events 66 | Total events 89 |
| PM anchored | 61% | 64% |
| CS anchored | 15% | 11% |
| Unanchored | 23% | 25% |
| . | Thrombin stimulation . | |
|---|---|---|
| 90 s, 0.1 U/mL . | 300 s, 0.1 U/mL . | |
| PM anchored | ||
| Single decondensed α-granule | 31% | 35% |
| N = 21 | N = 32 | |
| Compound fused decondensed α-granule string | 30% | 29% |
| N = 20 | N = 26 | |
| CS anchored | ||
| Single decondensed α-granule anchored to CS pore | 15% | 11% |
| N = 10 | N = 10 | |
| Unanchored | ||
| Single decondensed α-granule | 1% | 12% |
| N = 1 | N = 11 | |
| Compound fused decondensed α-granule string | 22% | 13% |
| N = 14 | N = 12 | |
| Relative frequency | Total events 66 | Total events 89 |
| PM anchored | 61% | 64% |
| CS anchored | 15% | 11% |
| Unanchored | 23% | 25% |
WT mouse platelets were stimulated with 0.1 U/mL thrombin for the indicated times. α-Granule fusion events were determined for all α-granules found in 10 randomly chosen platelets presenting in full platelet depth FIB-SEM image stacks at each time point. Granules were tracked at a nominal 5-nm Z-step resolution from image slices to score, in 3-dimensional space, whether the granule was anchored to a PM or CS pore complex. Image pixel size in XY was 5 nm × 5 nm.