GO functional analysis of upregulated genes
| Comparison . | Molecular function . | Cellular component . | Predicted regulators or transcription factors . |
|---|---|---|---|
| Untreated vs TPA+ (n = 1588) | Receptor binding (3.869E-18) | Cell surface (7.427E-25) | ETS (4. 039E-18) |
| Integrin binding (3.510E-10) | Integral component of plasma membrane (3.186E-23) | AP1 (5.373E-14) | |
| PU1 (1.352E-6) | |||
| BACH1 (2.647E-5) | |||
| Untreated vs MRTFAOETPA+ (n = 2014) | Receptor binding (5.415E-14) | Cell surface (1.076E-22) | ETS (2.646E-20) |
| Actin binding (4.831E-11) | Integral component of plasma membrane (2.313E-21) | AP1 (2.517E-12) | |
| SRF (9.979E-11) | |||
| TPA+ vs MRTFAOETPA+ (n = 2424) | Actin binding (2.135E-5) | Actin cytoskeleton (9.530E-7) | SRF (1.078E-10) |
| Cytoskeletal protein binding (1.065E-3) | Focal adhesion (2.590E-6) |
| Comparison . | Molecular function . | Cellular component . | Predicted regulators or transcription factors . |
|---|---|---|---|
| Untreated vs TPA+ (n = 1588) | Receptor binding (3.869E-18) | Cell surface (7.427E-25) | ETS (4. 039E-18) |
| Integrin binding (3.510E-10) | Integral component of plasma membrane (3.186E-23) | AP1 (5.373E-14) | |
| PU1 (1.352E-6) | |||
| BACH1 (2.647E-5) | |||
| Untreated vs MRTFAOETPA+ (n = 2014) | Receptor binding (5.415E-14) | Cell surface (1.076E-22) | ETS (2.646E-20) |
| Actin binding (4.831E-11) | Integral component of plasma membrane (2.313E-21) | AP1 (2.517E-12) | |
| SRF (9.979E-11) | |||
| TPA+ vs MRTFAOETPA+ (n = 2424) | Actin binding (2.135E-5) | Actin cytoskeleton (9.530E-7) | SRF (1.078E-10) |
| Cytoskeletal protein binding (1.065E-3) | Focal adhesion (2.590E-6) |
HEL cell TPA induction upregulates genes likely regulated by TCF factors (ETS, PU1), suggesting potential regulation via the SRF/TCF axis. With MRTFAOE, upregulated genes are significantly related to actin binding, and predicted regulators include both ETS factors (perhaps as part of the SRF/TCF complex) and SRF. When comparing TPA+ and MRTFAOETPA+ groups, actin cytoskeleton becomes the major GO term common to the upregulated genes, and SRF stands out as the only predicted regulatory transcription factor. Numbers in parentheses denote Bonferroni-corrected P values.