Frequency of cytogenetic aberrations separated by subclones and main clones
| Cytogenetic aberration . | PC-AL (n = 544) . | PC–non-AL (n = 519) . | P (PC-AL vs PC–non-AL) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| n/N (%) . | Subclone/main clone ratio . | n/N (%) . | Subclone/main clone ratio . | ||||||
| Main clone . | Main clone plus subclone . | Subclone . | Main clone . | Main clone plus subclone . | Subclone . | ||||
| t(11;14) | 328/544 (60.3) | 3/544 (0.6) | 0.009 | 75/519 (14.5) | 1/519 (0.2) | 0.013 | 1 | ||
| t(4;14) | 17/544 (3.1) | 2/544 (0.4) | 0.12 | 82/519 (15.8) | 4/519 (0.8) | 0.05 | 1 | ||
| t(14;16) | 13/543 (2.4) | 0/543 (0) | 0 | 30/511 (5.9) | 0/511 (0) | 0 | — | ||
| IgH unknown | 82/541 (15.2) | 12/541 (2.2) | 0.15 | 138/489 (28.2) | 28/489 (5.7) | 0.20 | 1 | ||
| Gain 1q21 | 102/544 (18.8) | 15/544 (2.8) | 50/544 (9.2) | 0.56 | 114/519 (22.0) | 35/519 (6.7) | 46/519 (8.9) | 0.54 | 1 |
| Gain 5p15/5q35 | 75/533 (14.1) | 1/533 (0.2) | 7/533 (1.3) | 0.11 | 111/367 (30.2) | 3/367 (0.8) | 22/367 (6.0) | 0.22 | 1 |
| Gain 9q34 | 123/543 (22.7) | 7/543 (1.3) | 42/543 (7.7) | 0.38 | 202/517 (39.0) | 23/517 (4.4) | 58/517 (11.2) | 0.36 | 1 |
| Gain 11q22.3 | 168/544 (30.9) | 12/544 (2.2) | 31/544 (5.7) | 0.24 | 179/519 (34.5) | 10/519 (1.9) | 35/519 (6.7) | 0.24 | 1 |
| Gain 11q23 | |||||||||
| Gain 15q22 | 74/542 (13.7) | 6/542 (1.1) | 17/542 (3.1) | 0.29 | 201/517 (38.9) | 14/517 (2.7) | 30/517 (5.8) | 0.20 | 1 |
| Gain 19q13 | 87/542 (16.1) | 5/542 (0.9) | 10/542 (1.8) | 0.16 | 191/519 (36.8) | 12/519 (2.3) | 55/519 (10.6) | 0.33 | .43 |
| Deletion 8p21 | 20/534 (3.7) | 18/534 (3.4) | 0.90 | 66/519 (12.7) | 42/519 (8.1) | 0.64 | 1 | ||
| Deletion 13q14 | 157/544 (28.9) | 52/544 (9.6) | 0.33 | 228/519 (43.9) | 36/519 (6.9) | 0.16 | 1 | ||
| Deletion 17p13 | 4/544 (0.7) | 12/544 (2.2) | 3.0 | 31/519 (6.0) | 16/519 (3.1) | 0.52 | 1 | ||
| Cytogenetic aberration . | PC-AL (n = 544) . | PC–non-AL (n = 519) . | P (PC-AL vs PC–non-AL) . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| n/N (%) . | Subclone/main clone ratio . | n/N (%) . | Subclone/main clone ratio . | ||||||
| Main clone . | Main clone plus subclone . | Subclone . | Main clone . | Main clone plus subclone . | Subclone . | ||||
| t(11;14) | 328/544 (60.3) | 3/544 (0.6) | 0.009 | 75/519 (14.5) | 1/519 (0.2) | 0.013 | 1 | ||
| t(4;14) | 17/544 (3.1) | 2/544 (0.4) | 0.12 | 82/519 (15.8) | 4/519 (0.8) | 0.05 | 1 | ||
| t(14;16) | 13/543 (2.4) | 0/543 (0) | 0 | 30/511 (5.9) | 0/511 (0) | 0 | — | ||
| IgH unknown | 82/541 (15.2) | 12/541 (2.2) | 0.15 | 138/489 (28.2) | 28/489 (5.7) | 0.20 | 1 | ||
| Gain 1q21 | 102/544 (18.8) | 15/544 (2.8) | 50/544 (9.2) | 0.56 | 114/519 (22.0) | 35/519 (6.7) | 46/519 (8.9) | 0.54 | 1 |
| Gain 5p15/5q35 | 75/533 (14.1) | 1/533 (0.2) | 7/533 (1.3) | 0.11 | 111/367 (30.2) | 3/367 (0.8) | 22/367 (6.0) | 0.22 | 1 |
| Gain 9q34 | 123/543 (22.7) | 7/543 (1.3) | 42/543 (7.7) | 0.38 | 202/517 (39.0) | 23/517 (4.4) | 58/517 (11.2) | 0.36 | 1 |
| Gain 11q22.3 | 168/544 (30.9) | 12/544 (2.2) | 31/544 (5.7) | 0.24 | 179/519 (34.5) | 10/519 (1.9) | 35/519 (6.7) | 0.24 | 1 |
| Gain 11q23 | |||||||||
| Gain 15q22 | 74/542 (13.7) | 6/542 (1.1) | 17/542 (3.1) | 0.29 | 201/517 (38.9) | 14/517 (2.7) | 30/517 (5.8) | 0.20 | 1 |
| Gain 19q13 | 87/542 (16.1) | 5/542 (0.9) | 10/542 (1.8) | 0.16 | 191/519 (36.8) | 12/519 (2.3) | 55/519 (10.6) | 0.33 | .43 |
| Deletion 8p21 | 20/534 (3.7) | 18/534 (3.4) | 0.90 | 66/519 (12.7) | 42/519 (8.1) | 0.64 | 1 | ||
| Deletion 13q14 | 157/544 (28.9) | 52/544 (9.6) | 0.33 | 228/519 (43.9) | 36/519 (6.9) | 0.16 | 1 | ||
| Deletion 17p13 | 4/544 (0.7) | 12/544 (2.2) | 3.0 | 31/519 (6.0) | 16/519 (3.1) | 0.52 | 1 | ||
Frequencies of each chromosomal aberration separately for subclones and main clones. The phenomenon main clone and subclone within a single chromosome, typically a mixed triploid and tetraploid state, is listed separately for chromosomal gains. The subclone/main clone ratio denotes the proportion of subclone vs main clone aberrations. Low ratios, such as 0.009 for t(11;14), indicate the cytogenetic aberrations are typically found as a main clone, whereas high ratios, such as 3.0 for deletion of 17p13, indicate that the aberrations are frequently of subclone size. For this analysis, the cases of main clone and subclone within a single chromosome were counted as both subclone and main clone aberrations. The P value in the last column addresses the subclone frequency difference between the PC-AL and PC–non-AL groups (again adjusted for the respective frequencies of MGUS/SMM/MM in both groups via logistic regression). P values were adjusted for multiple testing across probes using Holm correction to control the family-wise error rate.