Expression of immunoglobulin and immunoglobulin transcription factors in the tumor cells of classical and lymphocyte predominant Hodgkin disease
| . | HRS cells of classical HD . | L&H cells of LPHD . | Follicular lymphoma . |
|---|---|---|---|
| CD30 | 32/32 | 0/35 | 0/8 |
| CD20 | 6/32 | 35/35 | 8/8 |
| J-chain | 4*/32 | 31/35 | 6/8 |
| Immunoglobulin mRNA | 0/25† | 25/27 | 8/8 |
| Immunoglobulin protein | 0/25‡ | 26/32 | 8/8 |
| Oct2-mRNA | 21-153/11 | 6/6 | 4/4 |
| Oct2-protein | 41-154/321-155 | 35/35 | 8/8 |
| BOB.1 | 8/321-155 | 35/35 | 8/8 |
| . | HRS cells of classical HD . | L&H cells of LPHD . | Follicular lymphoma . |
|---|---|---|---|
| CD30 | 32/32 | 0/35 | 0/8 |
| CD20 | 6/32 | 35/35 | 8/8 |
| J-chain | 4*/32 | 31/35 | 6/8 |
| Immunoglobulin mRNA | 0/25† | 25/27 | 8/8 |
| Immunoglobulin protein | 0/25‡ | 26/32 | 8/8 |
| Oct2-mRNA | 21-153/11 | 6/6 | 4/4 |
| Oct2-protein | 41-154/321-155 | 35/35 | 8/8 |
| BOB.1 | 8/321-155 | 35/35 | 8/8 |
HRS cells indicates Hodgkin and Reed-Sternberg cells; HD, Hodgkin disease; LPHD, lymphocyte predominant Hodgkin disease; L&H cells, lymphocytic/histiocytic tumor cells of LPHD; Ig, immunoglobulin; mRNA, messenger RNA.
Very weak labeling.
One case with very weak signals for Igκ in some HRS cells.
Most cases showed in some or many HRS cells immunoreactivity with antibodies to IgG, Igκ, and Igλ. However, in the majority of the cases the immune reaction did not include the nuclear space of the HRS cells in contrast to the B cells and plasma cells that showed a strong nuclear space staining for one IgL chain type in all instances; in a few cases there was a nuclear space staining in some of the HRS cells.
These cases also expressed Oct2 at the protein level.
In only one case all HRS cells were positive.
There were no classical HD cases in which HRS cells expressed both Oct2 and BOB.1/OBF.1.