Table 1. Expression and function of... | American Society of Hematology

Table 1.

Expression and function of chemokine receptors in different subsets of NK cells

Receptor	Expression			Function
Receptor	NK	NA	AD	NK	NA	AD
CXCR1	no	no	low^*	no	no	no
CXCR2	no	no	no	no	no	no
CXCR3^‡	mod	mod^*	high^*	active	active	active^†
CXCR4	high	high	high	active	active	active
CXCR5	no	no	no	NT	NT	NT
CX₃CR1	no	no	high	no	no	active
CCR1^1-153	no	no	no	NT	NT	NT
CCR2	low	low	mod	active	active	active
CCR3	no	no	low	no	no	active
CCR4	high	high	high	active	active	active
CCR5	no	no	no	NT	NT	NT
CCR6	mod	mod	mod	active	active	active
CCR7	high	high	high	active	active	active
CCR8	no	no	high	no	no	active

Receptor	Expression			Function
Receptor	NK	NA	AD	NK	NA	AD
CXCR1	no	no	low^*	no	no	no
CXCR2	no	no	no	no	no	no
CXCR3^‡	mod	mod^*	high^*	active	active	active^†
CXCR4	high	high	high	active	active	active
CXCR5	no	no	no	NT	NT	NT
CX₃CR1	no	no	high	no	no	active
CCR1^1-153	no	no	no	NT	NT	NT
CCR2	low	low	mod	active	active	active
CCR3	no	no	low	no	no	active
CCR4	high	high	high	active	active	active
CCR5	no	no	no	NT	NT	NT
CCR6	mod	mod	mod	active	active	active
CCR7	high	high	high	active	active	active
CCR8	no	no	high	no	no	active

This table summarizes the results obtained regarding the surface expression of chemokine receptors as determined by flow cytometric assay, and functional analysis as determined by in vitro chemotaxis assay. NK cell populations used were nonactivated (designated as NK), those that are activated with IL-2 but did not adhere to plastic flasks (designated as NA), and those that are activated with IL-2 and adhered to plastic flasks (designated as AD).

The expression of chemokine receptors was classified as low (when the proportion of the positive cells is 5% or less); moderate (mod; when the proportion of positive cells is 5-10%); and high (when the proportion of positive cells is higher than 10%).

†

Active designation means that the receptor supports the chemotaxis response.

‡

The expression of CXCR3 on NK cells is considered moderate because between 5% and 12% of the cells were positive.

F1-153

Although flow cytometric analysis shows no expression of CCR1, mRNA for this receptor is expressed in the three NK cell subsets. The lack of surface expression may be due to CCR1 internalization.

No indicates no expression and/or no function; NT, not tested.

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