Screen engraftment analysis according to CD45, BFP, and CD34 expression
| Parameter . | Pools (number of mice) . | Negative CTRL (number of mice) . | Positive CTRL (number of mice) . |
|---|---|---|---|
| Stem screen | |||
| Total BM | |||
| >1% CD45+ | 69/77 (90%) | 8/11 (72%) | 8/8 (100%) |
| >1% BFP (CD45+) | 68/69 (99%) | 5/8 (63%) | 5/8 (63%) |
| FC (output/input) >1 | 31/68 (FC: 0.1-5.2; 46%) | 1/5 (FC: 0.2-1.3; 20%) | 2/5 (FC: 0.2-8.9, 40%) |
| Analyzed by ddPCR | 44 (∼2/3) | ||
| CD34+ | |||
| FC[BFP+(CD34+)/BFP+(CD45+)] | |||
| No BFP+ in CD34+ | 7 | 1 | 1 |
| FC < 0.95 | 38 | 2 | 3 |
| 0.95 < FC > 1.05 | 6 | 0 | 1 |
| FC> 1.05 | 17 | 2 | 0 |
| Analyzed by ddPCR | 12 | ||
| Progenitor screen | |||
| Total BM | |||
| >0.1% CD45+ (maximum 20%) | 53/64 (83%) | 5/8 (63%) | |
| >1% BFP(CD45+); (maximum 43%) | 41/53 (77%) | 1/5 (20%) | |
| FC (output/input) > 1 | 2/41 (5%) | ||
| FC (output/input) > 0.4 | 15/41 (37%) | 0/2 | |
| Analyzed by ddPCR | 18 (∼2/5) |
| Parameter . | Pools (number of mice) . | Negative CTRL (number of mice) . | Positive CTRL (number of mice) . |
|---|---|---|---|
| Stem screen | |||
| Total BM | |||
| >1% CD45+ | 69/77 (90%) | 8/11 (72%) | 8/8 (100%) |
| >1% BFP (CD45+) | 68/69 (99%) | 5/8 (63%) | 5/8 (63%) |
| FC (output/input) >1 | 31/68 (FC: 0.1-5.2; 46%) | 1/5 (FC: 0.2-1.3; 20%) | 2/5 (FC: 0.2-8.9, 40%) |
| Analyzed by ddPCR | 44 (∼2/3) | ||
| CD34+ | |||
| FC[BFP+(CD34+)/BFP+(CD45+)] | |||
| No BFP+ in CD34+ | 7 | 1 | 1 |
| FC < 0.95 | 38 | 2 | 3 |
| 0.95 < FC > 1.05 | 6 | 0 | 1 |
| FC> 1.05 | 17 | 2 | 0 |
| Analyzed by ddPCR | 12 | ||
| Progenitor screen | |||
| Total BM | |||
| >0.1% CD45+ (maximum 20%) | 53/64 (83%) | 5/8 (63%) | |
| >1% BFP(CD45+); (maximum 43%) | 41/53 (77%) | 1/5 (20%) | |
| FC (output/input) > 1 | 2/41 (5%) | ||
| FC (output/input) > 0.4 | 15/41 (37%) | 0/2 | |
| Analyzed by ddPCR | 18 (∼2/5) |