Screen details, input, and analysis
| . | Stem screen . | Progenitor screen . |
|---|---|---|
| Target cells | CB CD34+CD38− | CB CD34+CD38+ |
| Aimed transduction efficiency (mean achieved), % | 15 (13) | 25 (28) |
| Mouse strain | NSG | NSG-SGM3 |
| Total number of mice, sex, age, wk | 100 (96), male, 8 | 73, male and female, 8-16 |
| Group size, mice/pool | 5 (ctrl 4) | 4 |
| Cell count/ mouse, progeny plated | 10 000 | 21 000 |
| Controls | Negative: empty, LUC, gp91P415H;positive: DNAJB9, ID2 | Negative: LUC, gp91P415H, nontransduced (1×) |
| Analysis (BM: DNA, FACS), wk | 20 | 4.5 |
| FACS | CD45, BFP, CD34, viability | |
| . | Stem screen . | Progenitor screen . |
|---|---|---|
| Target cells | CB CD34+CD38− | CB CD34+CD38+ |
| Aimed transduction efficiency (mean achieved), % | 15 (13) | 25 (28) |
| Mouse strain | NSG | NSG-SGM3 |
| Total number of mice, sex, age, wk | 100 (96), male, 8 | 73, male and female, 8-16 |
| Group size, mice/pool | 5 (ctrl 4) | 4 |
| Cell count/ mouse, progeny plated | 10 000 | 21 000 |
| Controls | Negative: empty, LUC, gp91P415H;positive: DNAJB9, ID2 | Negative: LUC, gp91P415H, nontransduced (1×) |
| Analysis (BM: DNA, FACS), wk | 20 | 4.5 |
| FACS | CD45, BFP, CD34, viability | |