Table 3. Specificity of recombinant... | American Society of Hematology

Table 3.

Specificity of recombinant ADAMTS13 variants for VWF71 and multimeric VWF

Enzyme	Human VWF71 cleavage*			VWF multimer cleavage rate, normalized	Multimer/ VWF71 selectivity index†
Enzyme	Rate, min^–1	+mAbs, fold	+VWF D4, fold	VWF multimer cleavage rate, normalized	Multimer/ VWF71 selectivity index†
ADAMTS13	1.8 ± 0.04	5.5 ± 0.02	1.8 ± 0.3	100	54 ± 1.2
MDTCS	7.3 ± 0.1	0.98 ± 0.04	1.01 ± 0.01	25 ± 5	3.4 ± 0.5
MT7	9.0 ± 0.0	1.04 ± 0.01	0.98 ± 0.02	19 ± 4	2.1 ± 0.5
MT8	4.0 ± 0.1	1.2 ± 0.03	1.7 ± 0.01	42 ± 4	11 ± 1
delT2-7	4.7 ± 0.1	3.0 ± 0.1	0.94 ± 0.03	22 ± 3	4.8 ± 0.7
delT3-6	2.6 ± 0.1	2.5 ± 0.3	1.5 ± 0.02	59 ± 2	22 ± 0.9
Pigeon ADAMTS13	0.13 ± 0.01	1.04 ± 0.02	4.6 ± 0.3	11 ± 2	82 ± 14

Enzyme	Human VWF71 cleavage*			VWF multimer cleavage rate, normalized	Multimer/ VWF71 selectivity index†
Enzyme	Rate, min^–1	+mAbs, fold	+VWF D4, fold	VWF multimer cleavage rate, normalized	Multimer/ VWF71 selectivity index†
ADAMTS13	1.8 ± 0.04	5.5 ± 0.02	1.8 ± 0.3	100	54 ± 1.2
MDTCS	7.3 ± 0.1	0.98 ± 0.04	1.01 ± 0.01	25 ± 5	3.4 ± 0.5
MT7	9.0 ± 0.0	1.04 ± 0.01	0.98 ± 0.02	19 ± 4	2.1 ± 0.5
MT8	4.0 ± 0.1	1.2 ± 0.03	1.7 ± 0.01	42 ± 4	11 ± 1
delT2-7	4.7 ± 0.1	3.0 ± 0.1	0.94 ± 0.03	22 ± 3	4.8 ± 0.7
delT3-6	2.6 ± 0.1	2.5 ± 0.3	1.5 ± 0.02	59 ± 2	22 ± 0.9
Pigeon ADAMTS13	0.13 ± 0.01	1.04 ± 0.02	4.6 ± 0.3	11 ± 2	82 ± 14

Entries represent the mean ± standard error for at least 2 independent determinations.

The cleavage rate was determined in units of pmol product⋅pmol ADAMTS13⁻¹⋅min^–1, which reduces to min^–1.

Selectivity index, normalized VWF multimer cleavage rate/VWF71 cleavage rate.