Dependence of ADAMTS13 activity on VWF71 sequence
| Plasma species . | Cleavage of VWF71 substrates . | |
|---|---|---|
| Cattle/human, fold . | Armadillo/human, fold . | |
| Goat | 3.8 ± 0.9 | 0.8 ± 0.004 |
| Rat | 15 ± 0 | <LoD |
| Cattle | 6.5 ± 1 | <LoD |
| Sheep | 5.6 ± 0.2 | 0.34 ± 0.03 |
| Chicken | 1.8 ± 0.5 | 0.9 ± 0.1 |
| Horse | 3.4 ± 0.4 | 0.31 ± 0.03 |
| Recombinant horse | 2.4 ± 0.0 | 0.49 ± 0.02 |
| FVB mouse | 8.2 ± 1.5 | 0.18 ± 0.07 |
| Marmoset | 2.9 ± 0.03 | ND |
| Pig | 7.6 ± 0.2 | 0.34 ± 0.05 |
| Pigeon | 7.8 ± 0.2 | 0.13 ± 0.01 |
| Recombinant pigeon | 6.3 ± 0.5 | 0.42 ± 0.02 |
| C57BL/6 mouse | 8.2 ± 0.2 | 0.15 ± 0.01 |
| Frog | 7.6 ± 0.2 | 0.78 ± 0.01 |
| Baboon | 2.0 ± 0.05 | 0.2 ± 0.01 |
| Chimpanzee | 2.5 ± 0.0 | 0.28 ± 0.01 |
| Macaque | 2.8 ± 0.0 | 0.17 ± 0.01 |
| Ferret | 2.7 ± 0.2 | 0.28 ± 0.02 |
| Human | 2.5 ± 0.1 | 0.23 ± 0.01 |
| Recombinant human | 2.3 ± 0.1 | 0.27 ± 0.01 |
| Polar bear | 1.51 ± 0.00 | 0.31 ± 0.00 |
| Armadillo | 1.82 ± 0.10 | 0.98 ± 0.01 |
| Recombinant armadillo | 1.83 ± 0.00 | 0.71 ± 0.04 |
| Rabbit | 4.53 ± 0.01 | 0.38 ± 0.01 |
| Dog | 1.63 ± 0.03 | 0.59 ± 0.01 |
| Plasma species . | Cleavage of VWF71 substrates . | |
|---|---|---|
| Cattle/human, fold . | Armadillo/human, fold . | |
| Goat | 3.8 ± 0.9 | 0.8 ± 0.004 |
| Rat | 15 ± 0 | <LoD |
| Cattle | 6.5 ± 1 | <LoD |
| Sheep | 5.6 ± 0.2 | 0.34 ± 0.03 |
| Chicken | 1.8 ± 0.5 | 0.9 ± 0.1 |
| Horse | 3.4 ± 0.4 | 0.31 ± 0.03 |
| Recombinant horse | 2.4 ± 0.0 | 0.49 ± 0.02 |
| FVB mouse | 8.2 ± 1.5 | 0.18 ± 0.07 |
| Marmoset | 2.9 ± 0.03 | ND |
| Pig | 7.6 ± 0.2 | 0.34 ± 0.05 |
| Pigeon | 7.8 ± 0.2 | 0.13 ± 0.01 |
| Recombinant pigeon | 6.3 ± 0.5 | 0.42 ± 0.02 |
| C57BL/6 mouse | 8.2 ± 0.2 | 0.15 ± 0.01 |
| Frog | 7.6 ± 0.2 | 0.78 ± 0.01 |
| Baboon | 2.0 ± 0.05 | 0.2 ± 0.01 |
| Chimpanzee | 2.5 ± 0.0 | 0.28 ± 0.01 |
| Macaque | 2.8 ± 0.0 | 0.17 ± 0.01 |
| Ferret | 2.7 ± 0.2 | 0.28 ± 0.02 |
| Human | 2.5 ± 0.1 | 0.23 ± 0.01 |
| Recombinant human | 2.3 ± 0.1 | 0.27 ± 0.01 |
| Polar bear | 1.51 ± 0.00 | 0.31 ± 0.00 |
| Armadillo | 1.82 ± 0.10 | 0.98 ± 0.01 |
| Recombinant armadillo | 1.83 ± 0.00 | 0.71 ± 0.04 |
| Rabbit | 4.53 ± 0.01 | 0.38 ± 0.01 |
| Dog | 1.63 ± 0.03 | 0.59 ± 0.01 |
Cleavage of VWF71 substrates reported as the ratio of the rate of cleavage of cattle VWF71/the rate of cleavage of FRETS-VWF71 (middle column) or the rate of cleavage of armadillo VWF71/the rate of cleavage of FRETS-VWF71 (right column). Entries represent the mean ± standard error for at least 2 independent determinations.
<LoD, below limit of detection; ND, not done.