Table 1. Comparison of methods for blood... | American Society of Hematology

Table 1.

Comparison of methods for blood group antigen typing

Antibody-based typing	DNA-based typing
≤1-h turnaround	24-h turnaround
Manual	Automated
Fresh RBCs required	Any cell source*
Existing equipment	Specialized equipment and environment
Direct detection of antigen expression	Indirect “predicted” antigen expression
Interference from transfused RBCs or bound IgG	No interference from transfused RBCs or bound IgG
No reagents for some clinically significant antigens	Type for any antigen whose genetic basis is known
Weak/variable antigen expression may be missed	Detection of weak antigen expression
Low resolution	High resolution possible

Antibody-based typing	DNA-based typing
≤1-h turnaround	24-h turnaround
Manual	Automated
Fresh RBCs required	Any cell source*
Existing equipment	Specialized equipment and environment
Direct detection of antigen expression	Indirect “predicted” antigen expression
Interference from transfused RBCs or bound IgG	No interference from transfused RBCs or bound IgG
No reagents for some clinically significant antigens	Type for any antigen whose genetic basis is known
Weak/variable antigen expression may be missed	Detection of weak antigen expression
Low resolution	High resolution possible

IgG, immunoglobulin G.

Including fetal typing from cell-free DNA in maternal plasma.