Overview transplantation experiments
| Experiment . | Donor . | Recipient . | Vector (# batch no.) . | Transplanted population . | GFP+ at the day of transplantation, % . | No. of cells transplanted per mouse . | No. of mice . | No. of mice at time point of analysis† . |
|---|---|---|---|---|---|---|---|---|
| 1* | Bl6 (CD45.2) | MplKO (CD45.1) | PGK.GFP.pre (#1) | Bulk-transduced lin− | 37 | 5 × 105 | 3 | 3× > 16 wk |
| MplKO (CD45.2) | PGK.GFP.pre (#1) | 38 | 1.2 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Pbx1.iGFP.pre (#1) | 7 | 8.5 × 105 | 3 | 4× > 16 wk | |||
| MplKO (CD45.2) | PGK.Fzd4.iGFP.pre (#1) | 26 | 6.5 × 105 | 5 | 5× > 16 wk | |||
| 2* | MplKO (CD45.2) | PGK.GFP.pre (#2) | 41 | 5 × 105 | 3 | 3× > 16 wk | ||
| MplKO (CD45.2) | PGK.Hoxa10.iGFP.pre (#1) | 39 | 1.8 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#1) | 18 | 1.6 × 106 | 3 | 3× > 16 wk | |||
| 3* | Bl6 (CD45.2) | PGK.GFP.pre (#2) | 39 | 2 × 106 | 3 | 3× > 16 wk | ||
| MplKO (CD45.2) | PGK.Mycn.iGFP.pre (#1) | 19 | 2 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Pbx1.iGFP.pre (#1) | 8 | 106 | 7 | 7× > 16 wk | |||
| 4* | Bl6 (CD45.2) | PGK.GFP.pre (#3) | 35 | 1.5 × 106 | 6 | 2× 1 wk, 2× 4 wk, 2× > 16 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#3) | 36 | 1.5 × 106 | 6 | 2× 1 wk, 2× 4 wk, 2× > 16 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#2) | 8 | 106 | 2 | 2× > 16 wk | |||
| 5* | MplKO (CD45.2) | PGK.GFP.pre (#4) | 29 | 106 | 3 | 2× 1 wk, 1× > 16 wk | ||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#3) | 6 | 106 | 1 | >16 wk | |||
| 6 | MplKO (CD45.2) | PGK.GFP.pre (#4) | 33 | 106 | 2 | 1× 1 wk, 1× 4 wk | ||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#4) | 23 | 106 | 4 | 2× 1 wk, 2× 4 wk | |||
| 7 | Bl6 (CD45.2) | PGK.GFP.pre (#5) | 29 | 5 × 105 | 4 | 2× 1 wk, 2× 4 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#5) | 32 | 106 | 2 | 2× 4 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#5) | 19 | 106 | 3 | 1× 1 wk, 2× 4 wk | |||
| 8 | Bl6 (CD45.2) | PGK.GFP.pre (#6) | 41 | 1.3 × 106 | 5 | 5× > 16 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#6) | 42 | 1.3 × 106 | 3 | 3× > 16 wk |
| Experiment . | Donor . | Recipient . | Vector (# batch no.) . | Transplanted population . | GFP+ at the day of transplantation, % . | No. of cells transplanted per mouse . | No. of mice . | No. of mice at time point of analysis† . |
|---|---|---|---|---|---|---|---|---|
| 1* | Bl6 (CD45.2) | MplKO (CD45.1) | PGK.GFP.pre (#1) | Bulk-transduced lin− | 37 | 5 × 105 | 3 | 3× > 16 wk |
| MplKO (CD45.2) | PGK.GFP.pre (#1) | 38 | 1.2 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Pbx1.iGFP.pre (#1) | 7 | 8.5 × 105 | 3 | 4× > 16 wk | |||
| MplKO (CD45.2) | PGK.Fzd4.iGFP.pre (#1) | 26 | 6.5 × 105 | 5 | 5× > 16 wk | |||
| 2* | MplKO (CD45.2) | PGK.GFP.pre (#2) | 41 | 5 × 105 | 3 | 3× > 16 wk | ||
| MplKO (CD45.2) | PGK.Hoxa10.iGFP.pre (#1) | 39 | 1.8 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#1) | 18 | 1.6 × 106 | 3 | 3× > 16 wk | |||
| 3* | Bl6 (CD45.2) | PGK.GFP.pre (#2) | 39 | 2 × 106 | 3 | 3× > 16 wk | ||
| MplKO (CD45.2) | PGK.Mycn.iGFP.pre (#1) | 19 | 2 × 106 | 3 | 3× > 16 wk | |||
| MplKO (CD45.2) | PGK.Pbx1.iGFP.pre (#1) | 8 | 106 | 7 | 7× > 16 wk | |||
| 4* | Bl6 (CD45.2) | PGK.GFP.pre (#3) | 35 | 1.5 × 106 | 6 | 2× 1 wk, 2× 4 wk, 2× > 16 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#3) | 36 | 1.5 × 106 | 6 | 2× 1 wk, 2× 4 wk, 2× > 16 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#2) | 8 | 106 | 2 | 2× > 16 wk | |||
| 5* | MplKO (CD45.2) | PGK.GFP.pre (#4) | 29 | 106 | 3 | 2× 1 wk, 1× > 16 wk | ||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#3) | 6 | 106 | 1 | >16 wk | |||
| 6 | MplKO (CD45.2) | PGK.GFP.pre (#4) | 33 | 106 | 2 | 1× 1 wk, 1× 4 wk | ||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#4) | 23 | 106 | 4 | 2× 1 wk, 2× 4 wk | |||
| 7 | Bl6 (CD45.2) | PGK.GFP.pre (#5) | 29 | 5 × 105 | 4 | 2× 1 wk, 2× 4 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#5) | 32 | 106 | 2 | 2× 4 wk | |||
| MplKO (CD45.2) | PGK.Epcr.iGFP.pre (#5) | 19 | 106 | 3 | 1× 1 wk, 2× 4 wk | |||
| 8 | Bl6 (CD45.2) | PGK.GFP.pre (#6) | 41 | 1.3 × 106 | 5 | 5× > 16 wk | ||
| MplKO (CD45.2) | PGK.GFP.pre (#6) | 42 | 1.3 × 106 | 3 | 3× > 16 wk |
Mice from experiments 1 to 5 (analyzed at >16 weeks postprimary transplantation) were used as individual donors for secondary transplantation. A total of 107 total BM cells were transplanted into irradiated MplKO (CD45.1) recipients.
Indicated is the number of mice analyzed at the respective time points: at 1 week, 4 weeks, or >16 weeks after transplantation.