Table 1.

Primer sets used for PCR and targeted sequences for knockdown studies

Targeted sequencesForward primerReverse primer
STAT3-mut GCC​CCC​GCC​TCT​AGT​TCT​AGA​AAG​TTG​GCA-CCC​CGG​AAC​CTC​AGG​GGC​TG CAG​CCC​CTG​AGG​TTC​CGG​GGT​GCC​AAC​TT-TCT​AGA​ACT​AGA​GGC​GGG​GGC 
AP1_mut-1 ATC​CCA​TCC​ACT​TGG​CCC​TCC​AGC​CCG​ACT AGT​CGG​GCT​GGA​GGG​CCA​AGT​GGA​TGG​GAT 
AP1_mut-2 CGG​AAG​AGA​AAC​CGC​AGA​CCT​GCC​TGG​AAG AGT​CGG​GCT​GGA​GGG​CCA​AGT​GGA​TGG​GAT 
AP1_mut-3 AGG​AGA​CGG​AGG​TTA​CGG​GGA​GCC​GGT​TAG CTA​ACC​GGC​TCC​CCG​TAA​CCT​CCG​TCT​CCT 
AP1_mut-4 CCC​GCC​TCT​AGT​TCT​GTT​TCC​CGG​CAC​TGG CCA​GTG​CCG​GGA​AAC​AGA​ACT​AGA​GGC​GGG 
AP1_mut-5 CTC​GGG​CCC​GTG​CAG​CCA​CCA​CTT​CTC​TGG CCA​GAG​AAG​TGG​TGG​CTG​CAC​GGG​CCC​GAG 
AP1_mut-6 GTT​CCC​GGT​GGG​GGC​CGC​CGG​GTT​GGC​AGC GCT​GCC​AAC​CCG​GCG​GCC​CCC​ACC​GGG​AAC 
NF-kB_mut-1 TCG​GGC​CGC​GGA​GTT​GCC​AGA​AGG​GAG​GGG CCC​CTC​CCT​TCT​GGC​AAC​TCC​GCG​GCC​CGA 
NF-kB_mut-2 CGC​GCA​CAG​GGG​ACC​GCC​CGC​GAG​GGG​TCC GGA​CCC​CTC​GCG​GGC​GGT​CCC​CTG​TGC​GCG 
NF-kB_mut-3 GGA​GAC​GGA​ACG​TCG​GGT​AGC​CCG​GGG​GAT ATC​CCC​CGG​GCT​ACC​CGA​CGT​TCC​GTC​TCC 
NF-kB_mut-4 TGG​ATT​CCC​GGG​GAA​GGC​CCG​TGC​AGT​GAC GTC​ACT​GCA​CGG​GCC​TTC​CCC​GGG​AAT​CCA 
NF-kB_mut-5 GGA​TGG​GGC​TTC​ACA​GAA​CCC​GGG​ATT​AC-GGT​TCC​CGG​TGG​GGG​CCG​GGT​C GAC​CCG​GCC​CCC​ACC​GGG​AAC​CGT​AAT​CC-CGG​GTT​CTG​TGA​AGC​CCC​ATC​C 
HCK promoter cloning HCK-pro-F, GAG​CTG​GGC​ACA​ATC​CCA​TC HCK-In1-R, CAA​CTA​CCC​CCT​TCC​CCA​AGC​TG 
HCK promoter quantification HCK-pro-4F, ATC​CCT​TGA​TAG​GTG​GGG​TTA​GGA​T HCK-pro-4R, TAA​ACT​CAA​CTT​GCC​CCC​AAA​GAT​T 
PAX5 shRNA1 Target, GGT​AAT​TGG​AGG​ATC​CAA​A  
PAX5 shRNA2 Target, GCA​AGA​GAG​ACG​AAG​GTA​T  
JunB shRNA1 Target, CGC​ATC​AAA​GTG​GAG​CGC​AAG​C  
JunB shRNA2 Target, GGC​CTC​TCT​CTA​CAC​GAC​TAC​A  
Targeted sequencesForward primerReverse primer
STAT3-mut GCC​CCC​GCC​TCT​AGT​TCT​AGA​AAG​TTG​GCA-CCC​CGG​AAC​CTC​AGG​GGC​TG CAG​CCC​CTG​AGG​TTC​CGG​GGT​GCC​AAC​TT-TCT​AGA​ACT​AGA​GGC​GGG​GGC 
AP1_mut-1 ATC​CCA​TCC​ACT​TGG​CCC​TCC​AGC​CCG​ACT AGT​CGG​GCT​GGA​GGG​CCA​AGT​GGA​TGG​GAT 
AP1_mut-2 CGG​AAG​AGA​AAC​CGC​AGA​CCT​GCC​TGG​AAG AGT​CGG​GCT​GGA​GGG​CCA​AGT​GGA​TGG​GAT 
AP1_mut-3 AGG​AGA​CGG​AGG​TTA​CGG​GGA​GCC​GGT​TAG CTA​ACC​GGC​TCC​CCG​TAA​CCT​CCG​TCT​CCT 
AP1_mut-4 CCC​GCC​TCT​AGT​TCT​GTT​TCC​CGG​CAC​TGG CCA​GTG​CCG​GGA​AAC​AGA​ACT​AGA​GGC​GGG 
AP1_mut-5 CTC​GGG​CCC​GTG​CAG​CCA​CCA​CTT​CTC​TGG CCA​GAG​AAG​TGG​TGG​CTG​CAC​GGG​CCC​GAG 
AP1_mut-6 GTT​CCC​GGT​GGG​GGC​CGC​CGG​GTT​GGC​AGC GCT​GCC​AAC​CCG​GCG​GCC​CCC​ACC​GGG​AAC 
NF-kB_mut-1 TCG​GGC​CGC​GGA​GTT​GCC​AGA​AGG​GAG​GGG CCC​CTC​CCT​TCT​GGC​AAC​TCC​GCG​GCC​CGA 
NF-kB_mut-2 CGC​GCA​CAG​GGG​ACC​GCC​CGC​GAG​GGG​TCC GGA​CCC​CTC​GCG​GGC​GGT​CCC​CTG​TGC​GCG 
NF-kB_mut-3 GGA​GAC​GGA​ACG​TCG​GGT​AGC​CCG​GGG​GAT ATC​CCC​CGG​GCT​ACC​CGA​CGT​TCC​GTC​TCC 
NF-kB_mut-4 TGG​ATT​CCC​GGG​GAA​GGC​CCG​TGC​AGT​GAC GTC​ACT​GCA​CGG​GCC​TTC​CCC​GGG​AAT​CCA 
NF-kB_mut-5 GGA​TGG​GGC​TTC​ACA​GAA​CCC​GGG​ATT​AC-GGT​TCC​CGG​TGG​GGG​CCG​GGT​C GAC​CCG​GCC​CCC​ACC​GGG​AAC​CGT​AAT​CC-CGG​GTT​CTG​TGA​AGC​CCC​ATC​C 
HCK promoter cloning HCK-pro-F, GAG​CTG​GGC​ACA​ATC​CCA​TC HCK-In1-R, CAA​CTA​CCC​CCT​TCC​CCA​AGC​TG 
HCK promoter quantification HCK-pro-4F, ATC​CCT​TGA​TAG​GTG​GGG​TTA​GGA​T HCK-pro-4R, TAA​ACT​CAA​CTT​GCC​CCC​AAA​GAT​T 
PAX5 shRNA1 Target, GGT​AAT​TGG​AGG​ATC​CAA​A  
PAX5 shRNA2 Target, GCA​AGA​GAG​ACG​AAG​GTA​T  
JunB shRNA1 Target, CGC​ATC​AAA​GTG​GAG​CGC​AAG​C  
JunB shRNA2 Target, GGC​CTC​TCT​CTA​CAC​GAC​TAC​A  
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